陈春来 博士
研究员、博士生导师
1999-2003 学士 北京大学化学与分子工程学院
2003-2008 博士 北京大学化学与分子工程学院
2008-2015 博士后/研究员 University of Pennsylvania
2015-至今 研究员 清华大学生命科学学院，清华－北大生命科学联合中心（CLS）
研究兴趣、领域
1) 发展单分子荧光方法和技术；
2) CRISPR系列蛋白靶向和剪切机制的研究；
3) 核糖体在蛋白质合成过程中翻译和调控机制的研究。
代表性论文
1.Sijia Peng, Wenjuan Wang, and Chunlai Chen(*), (2018) Surface Transient Binding-Based Fluorescence Correlation Spectroscopy (STB-FCS), a Simple and Easy-to-Implement Method to Extend the Upper Limit of the Time Window to Seconds, J. Phys. Chem. B, Article ASAP, DOI: 10.1021/acs.jpcb.8b03476
2.Mengyi Yang(#), Sijia Peng(#), Ruirui Sun, Jingdi Lin, Nan Wang, and Chunlai Chen(*), (2018) The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET, Cell Reports, 22(2): 372-382
3.Sijia Peng, Wenjuan Wang (*) and Chunlai Chen(*), (2018) Breaking the concentration barrier for single molecule fluorescence measurements, Chemistry - A European Journal, 24: 1002-1009
4.Sijia Peng, Ruirui Sun, Wenjuan Wang and Chunlai Chen(*), (2017) Single-Molecule Photoactivation FRET: A General and Easy-to-Implement Approach to Break the Concentration Barrier, Angew. Chem. Int. Ed, 56(24):6882-6885
5.Sijia Peng, Mengyi Yang, Rui Ning Sun, Yang Liu, Wenjuan Wang, Qiaoran Xi, Haipeng Gong, and Chunlai Chen(*), (2017) Mechanism of actions of Oncocin, a proline-rich antimicrobial peptide, in early elongation revealed by single-molecule FRET, Protein & Cell, doi: 10.1007/s13238-017-0495-2
6.Sijia Peng, Ruirui Sun, Wenjuan Wang, and Chunlai Chen(*), (2017) Single-molecule FRET studies on interactions between elongation factor 4 (LepA) and ribosomes, Chinese Chemical Letters, doi: 10.1016/j.cclet.2017.12.006
7.Ryan M. Jamiolkowski, Chunlai Chen(*), Barry S. Cooperman(*), and Yale E. Goldman(*), (2017) tRNA Fluctuations Observed on Stalled Ribosomes Are Suppressed during Ongoing Protein Synthesis, Biophysical Journal, 113(11):2326-2335
8.Shan Sun, Lin Li, Fan Yang, Xiaojing Wang, Fenghui Fan, Mengyi Yang, Chunlai Chen, Xueming Li, Hong-Wei Wang, Sen-Fang Sui (*), (2017) Cryo-EM structures of the ATP-bound Vps4E233Q hexamer and its complex with Vta1 at near-atomic resolution, Nature Communications, Jul 17;8:16064
9.Chunlai Chen, Xiaonan Cui, John F. Beausang, Haibo Zhang, Ian Farrell, Barry S. Cooperman (*), and Yale E. Goldman (*), (2016) Elongation factor G initiates translocation through a power stroke, PNAS, 113(27):7515-20
10.Wenjuan Wang(*) and Chunlai Chen(*), (2016) Tracking translation of single mRNA molecule in live cells, Sci. Bull. 61(14):1071-1073
11.Chunlai Chen, Haibo Zhang, Steven L. Broitman, Michael Reiche, Ian Farrell, Barry S. Cooperman(*), and Yale E. Goldman(*), (2013) Dynamics of translation by single ribosomes through mRNA secondary structures, Nature Structural & Molecular Biology, 20, 582-588.
1.Sijia Peng, Wenjuan Wang, and Chunlai Chen(*), (2018) Surface Transient Binding-Based Fluorescence Correlation Spectroscopy (STB-FCS), a Simple and Easy-to-Implement Method to Extend the Upper Limit of the Time Window to Seconds, J. Phys. Chem. B, Article ASAP, DOI: 10.1021/acs.jpcb.8b03476
2.Mengyi Yang(#), Sijia Peng(#), Ruirui Sun, Jingdi Lin, Nan Wang, and Chunlai Chen(*), (2018) The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET, Cell Reports, 22(2): 372-382
3.Sijia Peng, Wenjuan Wang (*) and Chunlai Chen(*), (2018) Breaking the concentration barrier for single molecule fluorescence measurements, Chemistry - A European Journal, 24: 1002-1009
4.Sijia Peng, Ruirui Sun, Wenjuan Wang and Chunlai Chen(*), (2017) Single-Molecule Photoactivation FRET: A General and Easy-to-Implement Approach to Break the Concentration Barrier, Angew. Chem. Int. Ed, 56(24):6882-6885
5.Sijia Peng, Mengyi Yang, Rui Ning Sun, Yang Liu, Wenjuan Wang, Qiaoran Xi, Haipeng Gong, and Chunlai Chen(*), (2017) Mechanism of actions of Oncocin, a proline-rich antimicrobial peptide, in early elongation revealed by single-molecule FRET, Protein & Cell, doi: 10.1007/s13238-017-0495-2
6.Sijia Peng, Ruirui Sun, Wenjuan Wang, and Chunlai Chen(*), (2017) Single-molecule FRET studies on interactions between elongation factor 4 (LepA) and ribosomes, Chinese Chemical Letters, doi: 10.1016/j.cclet.2017.12.006
7.Ryan M. Jamiolkowski, Chunlai Chen(*), Barry S. Cooperman(*), and Yale E. Goldman(*), (2017) tRNA Fluctuations Observed on Stalled Ribosomes Are Suppressed during Ongoing Protein Synthesis, Biophysical Journal, 113(11):2326-2335
8.Shan Sun, Lin Li, Fan Yang, Xiaojing Wang, Fenghui Fan, Mengyi Yang, Chunlai Chen, Xueming Li, Hong-Wei Wang, Sen-Fang Sui (*), (2017) Cryo-EM structures of the ATP-bound Vps4E233Q hexamer and its complex with Vta1 at near-atomic resolution, Nature Communications, Jul 17;8:16064
9.Chunlai Chen, Xiaonan Cui, John F. Beausang, Haibo Zhang, Ian Farrell, Barry S. Cooperman (*), and Yale E. Goldman (*), (2016) Elongation factor G initiates translocation through a power stroke, PNAS, 113(27):7515-20
10.Wenjuan Wang(*) and Chunlai Chen(*), (2016) Tracking translation of single mRNA molecule in live cells, Sci. Bull. 61(14):1071-1073
11.Chunlai Chen, Haibo Zhang, Steven L. Broitman, Michael Reiche, Ian Farrell, Barry S. Cooperman(*), and Yale E. Goldman(*), (2013) Dynamics of translation by single ribosomes through mRNA secondary structures, Nature Structural & Molecular Biology, 20, 582-588.
联系方式
E-mail:chunlai@mail.tsinghua.edu.cn
