葛亮 博士

研究员,助理教授


2001-2005年,山东师范大学生命科学院                  学士

2005-2011年,中科院上海生化细胞所                      生物化学博士

2011-2015年,美国加州大学伯克利分校                  博士后

2015-2017年,美国加州大学伯克利分校                  研究员(Research Specialist)

2017-               清华大学生命科学学院-生命科学联合中心  助理教授,  研究员

 

●  研究兴趣、领域:


生命的本质之一是生物体独立于外界建立自我维持的代谢稳态调节环境,并能应对和抵抗外界压力和刺激。我们致力于解答“细胞在外界压力下如何维持内部稳态平衡?”这一细胞生物学的本质问题。我们研究主要集中在三个相互关联的细胞学过程:细胞自噬(应激状态下细胞自我清理和更新内部物质的过程)、非经典分泌(近来发现的应激状态下不依赖于传统内质网高尔基体运输的分泌过程)和细胞器互作(细胞器之间通过接触相互协作调控细胞生命活动和应激的过程)。我们运用细胞生物学、生物化学、动物模型和计算生物学等手段,研究这三个过程的细胞内调节和相互作用的分子机制,以及其生物学意义和相关人类疾病。

●  代表性论文:


1. Min Zhang, Lei Liu, Xubo Lin, Yang Wang, Qing Guo, Ying Li, Shulin Li, Yuxin Sun, Di Zhang, Xiachen Lv, Li Zheng and Liang Ge*. A translocation pathway for vesicle-mediated unconventional protein secretion. Cell (2020) 181:637-652. https://doi.org/10.1016/j.cell.2020.03.031
2. Min Zhang, and
Liang Ge*. Cell-Free Reconstitution of Autophagic Membrane Formation. Methods Mol Biol (2019), 1880:135-148. doi: 10.1007/978-1-4939-8873-0_7.
3. Min Zhang, Yu Wang, and
Liang Ge*. Endomembrane remodeling in autophagic membrane formation. Autophagy (2018);14(5):918-920.
4. Min Zhang, Dawei Liu, and
Liang Ge*. In vitro dissection of autophagy. Curr Protoc Cell Biol (2017), 77, 11.23.1–11.23.17. doi: 10.1002/cpcb.3

5. Liang Ge*, Min Zhang, Sam Kenny, Anandita Mathur, Dawei Liu, Miharu Maeda, Kota Saito, Ke Xu, and Randy Schekman. Remodeling of ER-exit sites initiates a membrane supply pathway for autophagosome biogenesis. EMBO Report (2017) 18(9):1586-1603. * Corresponding author.

6. Livia W Brier, Min Zhang, Liang Ge*. Mechanistically dissecting autophagy: insights from in vitro reconstitution. J Mol Biol (2016) 8;428(9 Pt A):1700-13.*Corresponding author

7. Liang Ge, Livia Wilz and Randy Schekman. Biogenesis of autophagosomal precursors for LC3 lipidation from the ER-Golgi intermediate compartment. Autophagy (2015) 11(12):2372-4

8. Liang Ge, Min Zhang and Randy Schekman. Phosphatidylinositol 3-kinase and COPII generate LC3 lipidation vesicles from the ER-Golgi intermediate compartment. eLife (2014) Nov 28; 3:e04135.

9. Liang Ge, Sulochanadevi Baskaran, Randy Schekman and James H Hurley. The protein-vesicle network of autophagy. Curr Opin Cell Biol (2014) 29C:18-24.

10. Liang Ge and Randy Schekman. The ER-Golgi intermediate compartment feeds the phagophore membrane. Autophagy (2013) 10(1):170-2

11. Liang Ge, David Melville, Min Zhang and Randy Schekman. The ER-Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis. eLife (2013) Aug 6; 2:e00947. Selected for F1000Prime and highlighted by Daniel Klionsky [Amélie Bernard and Daniel J Klionsky. Defining the membrane precursor supporting the nucleation of the phagophore. Autophagy (2014) 10(1):1-2].

12. Liang Ge, Wei Qi, Li-Juan Wang, Hong-Hua Miao, Yu-Xiu Qu, Bo-Liang Li and Bao-Liang Song. Flotillins play an essential role in Niemann-Pick C1 Like 1-mediated cholesterol uptake. Proc Natl Acad Sci USA (2011) 108(2):551-556

13. Liang Ge, Jing Wang, Wei Qi, Hong-Hua Miao, Jian Cao, Yu-Xiu Qu, Bo-Liang Li and Bao-Liang Song. The cholesterol absorption inhibitor Ezetimibe acts by blocking the sterol-induced internalization of NPC1L1. Cell Metabolism (2008) 7:508-519. Selected for F1000Prime; Featured Article; Commented in Cell Metabolism (2008) 7:469-471

14. Jin-Hui Zhang*,Liang Ge*, Wei Qi, Liqing Zhang, Hong-Hua Miao, Bo-Liang Li, Maojun Yang and Bao-Liang Song. The N-terminal domain of NPC1L1 protein binds cholesterol and plays essential roles in cholesterol uptake. J Biol Chem (2011) 286(28):25088-097            *Equal contribution

15. Bei-Bei Chu*, Liang Ge*, Yang Zhao, Jing Wang, Bo-Liang Li and Bao-Liang Song. Requirement of myosin Vb·Rab11a·Rab11-FIP2 complex in cholesterol-regulated translocation of NPC1L1 to the cell surface. J Biol Chem (2009) 284(33):22481-90.  *Equal contribution

 


●  荣誉、奖励及参加学术团体的情况:


NIH Pathway to Independence Award (K99/R00) (2015-2017)

Jane Coffin Child Foundation Fellowship (2013-2015)

Human Frontier Science Program Fellowship (2012)

 

●  联系方式:


电话:86-10-62789019 (实验室)      

E-mail: liangge@mail.tsinghua.edu.cn