Haifeng WANG, Ph.D.
Associate Professor
2000-2004 B.S. China Agricultural University
2004-2007 M.S. Tsinghua University
2007-2013 Ph.D, University of California, Davis, USA
2013-2014 Postdoctoral researcher, Stanford University, USA
2021-2025 Assistant Professor, School of Life Sciences, Tsinghua University
2025-present Associate Professor, School of Life Sciences, Tsinghua University
2021-present Principal investigator, Center for Life Sciences, Tsinghua University
Research interest
We are interested in developing and using CRISPR technologies to study cell and developmental biology and to address disease-related mechanism, and applying these tools to medical diagnosis and therapy. We mainly focus on:
(1) Developing CRISPR tools to study the regulatory mechanisms of 3D genome and transcriptome in living cells.
(2) Applying new tools to investigate the spatiotemporal dynamics of the 3D genome and transcriptome and to understand the mechanism of gene regulation during disease and development.
(3) Precision medicine: developing diagnostic and therapeutical approaches for diseases related to genomic or transcriptome abnormalities.
Selected publications
1. Liu M, Huang K, Zhang J, Li Q, Wang X, Gu B, Tang H, Du Z, Hu L, Qi S, Ma Y, Yu H, Xie W*, Fang X*, Wang H*. CRISPR live-cell imaging reveals chromatin dynamics and enhancer interactions at multiple non-repetitive loci, Nature Biotechnology, 2025.
2. Chen Q, Wang S, Zhang J, Xie M, Lu B, He J, Zhen Z, Li J, Zhu J, Li R, Li P, Wang H, Vakoc C, Roeder R*, Chen M*. JMJD1C forms condensate to facilitate a RUNX1-dependent gene expression program shared by multiple types of AML cells Protein & Cell, 2025 16-5, 338-364.
3. Du Z, Hu L, Zou Z, Liu M, Li Z, Lu X, Harris C, Xiang Y, Chen F, Yu G, Xu K, Kong F, Xu Q, Huang B, Liu L, Fan Q, Wang H*, Kalantry S*, Xie W*. Stepwise de novo establishment of inactive X chromosome architecture in early development, Nature Genetics, 2024 56, 2185–2198.
4. Yang L, Min Y, Liu Y, Gao B, Liu X, Huang Y, Wang H, Yang L, Liu Z, Chen L*. CRISPR-array-mediated imaging of non-repetitive and multiplex genomic loci in living cells, Nature Methods, 2024 21, 1646–1657.
5. Zhang Z, Rong X, Xie T, Li Z, Song H, Zhen S, Wang H, Wu J, Jaffrey S, Li X*. Fluorogenic CRISPR for genomic DNA imaging, Nature Communications, 2024 15, 934.
6. Li Q, Gao Y, Wang H*. CRISPR-Based Tools for Fighting Rare Diseases, Life, 2022 Nov 24; 12(12):1968.
7. Lin X, Liu Y, Liu S, Zhu X, Wu L, Zhu Y, Zhao D, Xu X, Chemparathy A, Wang H, Cao Y, Nakamura M, Noordermeer JN, La Russa M, Wong WH, Zhao K, Qi LS*. Nested epistasis enhancer networks for robust genome regulation, Science, 2022 Aug 11; Vol 377, Issue 6610, pp. 1077-1085.
8. Wang H, Han M, Qi LS, Engineering 3D genome organization, Nature Reviews Genetics, 2021 Feb; PMID: 33558716;
9. Wang H, Nakamura M, Abbott TR, Zhao D, Luo K, Yu C, Nguyen CM, Lo A, Daley PT, La Russa M, Liu Y, Qi LS, CRISPR-mediated live imaging of genome editing and transcription. Science, 2019 Sep; Vol. 365, Issue 6459, pp. 1301-1305.
10. Wang H, Xu X, Nguyen CM, Liu Y, Gao Y, Lin X, Daley PT, Kipniss NH, La Russa M, Qi LS, CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear Organization. Cell, 2018 Oct; 175, 1405-1417 e1414.
11. Liu Y, Yu C, Daley T, Wang F, Cao W, Lin X, Still C, Bhate S, Liu H, Zhao D, Wang H, Xie X, Ding S, Wong WH, Wernig M, Qi LS, CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming. Cell Stem Cell, 2018 Nov; 23(5):758-771.e8.
12. Kipniss N, Dingal PCD, Abbott T, Gao Y, Wang H, Dominguez A, and Labanieh L, Qi LS, Engineering Cell Sensing and Responses Using a GPCR-Coupled CRISPR-Cas System. Nature Communications, 2017 Dec; 2212.
13. Wang H, La Russa M, Qi LS, CRISPR-Cas9 in genome editing and beyond. The Annual Review of Biochemistry, 2016 Aug; Vol. 85: 227-264.
Contact information
Email：hfwang(at)mail.tsinghua.edu.cn
Lab Website：http://www.wanglab-thu.org.cn/
