Hongwei Wang     Ph.D.


1992-1996    B.S., Department of Biological Sciences and Biotechnology, Tsinghua University

1996-2001    Ph.D., Department of Biological Sciences and Biotechnology, Tsinghua University

2001-2006    Postdoctoral Fellow, Life Sciences Division, Lawrence Berkeley National Laboratory, USA

2006-2008    Research Scientist, Life Sciences Division, Lawrence Berkeley National Laboratory, USA

2009-2011    Tenure-Track Assistant Professor, Department of Molecular Biophysics and Biochemistry, Yale University, USA

2010.12-present      Professor, School of Life Sciences, Tsinghua University

2016.04-2021.04      Dean, School of Life Sciences, Tsinghua University


Research interest

Our research group is devoted to elucidating the architecture and mechanism of macromolecular complexes and assemblies with cryo-electron microscopy. More specifically, we are interested in:

1.  Methodology development for more efficient and high resolution cryo-electron microscopy;

2. The mechanism and regulations of nucleic acid quality control;

3. The coordination mechanisms of cytoskeleton and membrane systems.

Selected publications

1. Zhou J, Wang A, Song Y, Liu N, Wang J, Li Y, Liang X, Li GH, Chu HY, Wang HW*. (2023) Structural insights into the mechanism of GTP initiation of microtubule assembly. Nat. Commun., 14(1):5980.

2. Cheng H, Zheng LM, Liu N, Huang CY, Xu J, Lu Y, Cui XY, Xu K, Hou Y, Tang JC, Zhang Z, Li J, Ni XD, Chen YN*, Peng HL*, Wang HW*. (2023) Dual-Affinity Graphene Sheets for High-Resolution Cryo-Electron Microscopy. J. Am. Chem. Soc., 145(14):8073-8081.

3. You X, Zhang X, Cheng J, Xiao YN, Ma JF, Sun S, Zhang XZ*, Wang HW*, Sui SF*. (2023) In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex. Nature, 616(7955):199-206.

4. Zheng LM, Liu N*, Gao XY, Zhu WQ, Liu K, Wu C, Yan R, Zhang JC, Gao X, Yao YT, Deng B, Xu J, Lu Y, Liu, ZM, Li MS, Wei XD*, Wang HW*, Peng HL*. (2023) Uniform thin ice on ultraflat graphene for high-resolution cryo-EM. Nat Methods, 20(1):123-130.

5. Lu Y, Liu N*, Liu Y, Zheng LM, Yang JH, Wang J, Jia X, Zi QR, Peng HL*, Rao Y*, Wang HW*. (2022) Functionalized graphene grids with various charges for single-particle cryo-EM. Nat. Commun., 13(1):6718.

6. Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li XM, Huang Y, Wang HW*, Ma JB*. (2022) Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD. Nature, 607(7918):399-406.

7. Zheng L, Liu N, Liu Y, Li N, Zhang J, Wang C, Zhu W, Chen Y, Ying D, Xu J, Yang Z, Gao X, Tang J, Wang X, Liang Z, Zou R, Li Y, Gao P, Wei X*, Wang HW*, Peng HL*. (2021) Atomically Thin Bilayer Janus Membranes for Cryo-electron Microscopy. ACS Nano, 15(10):16562-16571.

8. Liu N, Zheng LM, Xu J, Wang J, Hu CX, Lan J, Zhang X, Zhang J, Xu K, Cheng H, Yang Z, Gao X, Wang X, Peng HL*, Chen YN*, Wang HW*. (2021) Reduced graphene oxide membrane as supporting film for high-resolution cryo-EM. Biophysics Reports, 7(3): 227-238. (Cover)

9. Jin W, Wang J, Liu CP, Wang HW*, Xu RM*. (2020) Structural Basis for pri-miRNA Recognition by Drosha. Mol. Cell, 78(3): 423-439.

10. Zheng L, Chen Y, Li N, Zhang J, Liu N, Liu J, Dang W, Deng B, Li Y, Gao X, Tan C, Yang Z, Xu S, Wang M, Yang H, Sun L, Cui Y, Wei X, Gao P*, Wang HW*, Peng H*. (2020) Robust ultraclean atomically thin membranes for atomic-resolution electron microscopy. Nat. Commun., 11(1):541.

11. Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J*, Wang HW*. (2019) Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution. Nat. Commun., 10(1):2386.

12. Liu N, Zhang J, Chen Y*, Liu C, Zhang X, Xu K, Wen J, Luo Z, Chen S, Gao P, Jia K, Liu Z, Peng H*, Wang HW*. (2019) Bioactive Functionalized Monolayer Graphene for High-Resolution Cryo-Electron Microscopy. J. Am. Chem. Soc., 141(9):4016-4025.

13. Wang J, Wang J, Hu M, Wu S, Qi J, Wang G, Han Z, Qi Y, Gao N, Wang HW*, Zhou JM*, Chai J*. (2019) Ligand-triggered allosteric ADP release primes a plant NLR complex. Science, 364(6435). pii: eaav5868.

14. Wang J, Hu M, Wang J, Qi J, Han Z, Wang G, Qi Y, Wang HW*, Zhou JM*, Chai J*. (2019) Reconstitution and structure of a plant NLR resistosome conferring immunity. Science, 364(6435). pii: eaav5870.

15. Liu Z, Wang J, Cheng H, Ke X, Sun L, Zhang Q, Wang HW*. (2018) Cryo-EM Structure of Human Dicer and Its Complexes with a Pre-miRNA Substrate. Cell, 173(5):1191-1203.


Contact information

Tel: +86-10-62772390 (office)

+86-10-62798651 (lab)

E-mail: hongweiwang@tsinghua.edu.cn

Lab website: http://cryoem.life.tsinghua.edu.cn/