Hongwei Wang Ph.D.
Professor
1992-1996 B.S., Department of Biological Sciences and Biotechnology, Tsinghua University
1996-2001 Ph.D., Department of Biological Sciences and Biotechnology, Tsinghua University
2001-2006 Postdoctoral Fellow, Life Sciences Division, Lawrence Berkeley National Laboratory, USA
2006-2008 Research Scientist, Life Sciences Division, Lawrence Berkeley National Laboratory, USA
2009-2011 Tenure-Track Assistant Professor, Department of Molecular Biophysics and Biochemistry, Yale University, USA
2010.12-present Professor, School of Life Sciences, Tsinghua University
2016.04-2021.04 Dean, School of Life Sciences, Tsinghua University
Research interest
Our research group is devoted to elucidating the architecture and mechanism of macromolecular complexes and assemblies with cryo-electron microscopy. More specifically, we are interested in:
1. Methodology development for more efficient and high resolution cryo-electron microscopy;
2. The mechanism and regulations of nucleic acid quality control;
3. The coordination mechanisms of cytoskeleton and membrane systems.
Selected publications
1. Cao N, Wang J*, Deng T, Fan B, Su S, Ma JB*, Wang HW*. (2025) Structural basis of endo-siRNA processing by Drosophila Dicer-2 and Loqs-PD. Nucleic Acids Res., 53(4):gkaf102.
2. Zheng, LM, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N*, Wang HW*, Peng HL*. (2024) Self-assembled superstructure alleviates air-water interface effect in cryo-EM. Nat Commun., 15(1):7300.
3. Yang Z, Fan J, Wang J, Fan X, Ouyang Z*, Wang HW*, Zhou XY*. (2024) Electrospray-assisted cryo-EM sample preparation to mitigate interfacial effects. Nat Methods, 21(6):1023-1032.
4. Xu J, Gao XY, Zheng LM, Jia X, Xu K, Ma Y, Wei XD, Liu N*, Peng HL*, Wang HW*. (2024) Graphene sandwich-based biological specimen preparation for cryo-EM analysis. Proc Natl Acad. Sci USA, 121(5):e2309384121.
5. Zhou J, Wang A, Song Y, Liu N, Wang J, Li Y, Liang X, Li GH, Chu HY, Wang HW*. (2023) Structural insights into the mechanism of GTP initiation of microtubule assembly. Nat. Commun., 14(1):5980.
6. You X, Zhang X, Cheng J, Xiao YN, Ma JF, Sun S, Zhang XZ*, Wang HW*, Sui SF*. (2023) In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex. Nature, 616(7955):199-206.
7. Zheng LM, Liu N*, Gao XY, Zhu WQ, Liu K, Wu C, Yan R, Zhang JC, Gao X, Yao YT, Deng B, Xu J, Lu Y, Liu, ZM, Li MS, Wei XD*, Wang HW*, Peng HL*. (2023) Uniform thin ice on ultraflat graphene for high-resolution cryo-EM. Nat Methods, 20(1):123-130.
8. Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li XM, Huang Y, Wang HW*, Ma JB*. (2022) Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD. Nature, 607(7918):399-406.
9. Liu N, Zheng LM, Xu J, Wang J, Hu CX, Lan J, Zhang X, Zhang J, Xu K, Cheng H, Yang Z, Gao X, Wang X, Peng HL*, Chen YN*, Wang HW*. (2021) Reduced graphene oxide membrane as supporting film for high-resolution cryo-EM. Biophysics Reports, 7(3): 227-238. (Cover)
10. Jin W, Wang J, Liu CP, Wang HW*, Xu RM*. (2020) Structural Basis for pri-miRNA Recognition by Drosha. Mol. Cell, 78(3): 423-439.
11. Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J*, Wang HW*. (2019) Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution. Nat. Commun., 10(1):2386.
12. Liu N, Zhang J, Chen Y*, Liu C, Zhang X, Xu K, Wen J, Luo Z, Chen S, Gao P, Jia K, Liu Z, Peng H*, Wang HW*. (2019) Bioactive Functionalized Monolayer Graphene for High-Resolution Cryo-Electron Microscopy. J. Am. Chem. Soc., 141(9):4016-4025.
13. Wang J, Wang J, Hu M, Wu S, Qi J, Wang G, Han Z, Qi Y, Gao N, Wang HW*, Zhou JM*, Chai J*. (2019) Ligand-triggered allosteric ADP release primes a plant NLR complex. Science, 364(6435). pii: eaav5868.
14. Wang J, Hu M, Wang J, Qi J, Han Z, Wang G, Qi Y, Wang HW*, Zhou JM*, Chai J*. (2019) Reconstitution and structure of a plant NLR resistosome conferring immunity. Science, 364(6435). pii: eaav5870.
15. Liu Z, Wang J, Cheng H, Ke X, Sun L, Zhang Q, Wang HW*. (2018) Cryo-EM Structure of Human Dicer and Its Complexes with a Pre-miRNA Substrate. Cell, 173(5):1191-1203.
Contact information
Tel: +86-10-62772390 (lab)
+86-10-62798651 (office)
E-mail: hongweiwang@tsinghua.edu.cn
Lab website: http://cryoem.life.tsinghua.edu.cn/
