Jun-Jie (Gogo) Liu Ph.D.
Associate Professor
2007-2011 B.S., School of Life Sciences, Sichuan University
2011-2016 Ph.D., School of Life Sciences, Tsinghua University
2016-2018 Postdoctoral Fellow, MBIB Division, Lawrence Berkeley National Laboratory
2018-2020 Postdoctoral Fellow, Department of Molecular and Cell Biology, UC Berkeley
2018-2020 Life Science Research Fund Fellow Sponsored by Pfizer
2020-present Assistant professor, Associate professor, School of Life Sciences, Tsinghua University
2020-present PI, Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University
Beijing Frontier Research Center for Biological Structure, Tsinghua University
● Research Interest：
Gogo's laboratory combines bioinformatics, biochemistry, biophysics, and cell biology methods to conduct research in the following directions:
(1) Design and development of novel gene editing tools
(2) Reveal the molecular details of the RNA-involved nuclease machinery
In recent years, Gogo lab has created several compact genome editors such as CRISPR-Casx and CasPi (Mol. Cell, 2022; Cell Res., 2023), discovered the pro-CRISPR system (Nature, 2024), developed HYER ribozymes which can hydrolytically cut the DNA (Science, 2024), and revealed the molecular mechanism of RNA-governed retrotransposition by R2 elements (Cell, 2023).
● Selected Publications:
1. Zhang SY#, Sun A#, Qian JM#, Lin S#, Xing W, Yang Y, Zhu HZ, Zhou XY, Guo YS, Liu Y, Meng Y, Jin SL, Song W, Li CP, Li Z, Jin S, Wang JH, Dong MQ, Gao C, Chen C*, Bai Y*, Liu JJ*. Pro-CRISPR PcrIIC1 associated Cas9 system for enhanced bacterial immunity. Nature, 630, 484–492 (2024).
2. Liu ZX#, Zhang S#, Zhu HZ#, Chen ZH#, Yang Y#, Li LQ#, Lei Y, Liu Y, Li DY, Sun A, Li CP, Tan SQ, Wang GL, Shen JY, Jin S, Gao C, Liu JJ*. Hydrolytic endonucleolytic ribozyme (HYER) is programmable for sequence-specific DNA cleavage. Science, 2024 Feb 2; 383(6682): eadh4859.
3. Deng P#, Tan SQ#, Yang QY, Fu L, Wu Y, Zhu HZ, Sun L, Bao Z, Lin Y, Zhang QC, Wang H, Wang J*, Liu JJ*. Structural RNA components supervise the sequential DNA cleavage in R2 retrotransposon. Cell, 2023 Jun 22;186(13):2865-2879.
4. Li DY#, Li LQ#, Liu JJ*. Nucleases in gene-editing technologies: past and prologue. National Science Open, 2023 Jul 18;2(5): 20220067.
5. Hu Z#, Sun A#, Yang J, Naz G, Sun G, Li Z, Liu JJ*, Zhang S*, Zhang X*. Regulation of the CRISPR-Cas12a system by methylation and demethylation of guide RNA. Chemical Science, 2023,14(22):5945-5955.
6. Sun A#, Li CP#, Chen Z#, Zhang S#, Li DY, Yang Y, Li LQ, Zhao Y, Wang K, Li Z, Liu J, Liu S, Wang J*, Liu JJ*. The compact Casπ (Cas12l) ‘bracelet’ provides a unique structural platform for DNA manipulation. Cell Research, 2023 Mar;33(3), 229–244.
7. Tsuchida CA#, Zhang S#, Doost MS#, Zhao Y#, Wang J, O'Brien E, Fang H, Li CP, Li D, Hai ZY, Chuck J, Brötzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA*, Liu JJ*. Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity. Mol Cell., 2022 Mar 17;82(6):1199-1209.
8. Yang M, Sun R, Deng P, Yang Y, Wang W, Liu JJ*, Chen C*. Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search. Chem Sci., 2021 Aug 20;12(38):12776-12784.
9. Liu JJ*, Wang HW*. Cryo-Electron Microscopy of Endogenous Yeast Exosomes. Methods in Molecular Biology, 2020;2062:401-415. (Book Chapter)
10. Liu TY#, Liu JJ#, Aditham AJ, Nogales E, Doudna JA*. Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble. Nat Commun., 2019 Jul 5;10(1):3001.
11. Liu JJ#, Orlova N#, Oakes BL#, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E*, Doudna JA*. Author Correction: CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature, 2019 Apr;568(7752):E8-E10.
12. Jiang F#, Liu JJ#, Osuna BA#, Xu M, Berry JD, Rauch BJ, Nogales E, Bondy-Denomy J*, Doudna JA*. Temperature-Responsive Competitive Inhibition of CRISPR-Cas9. Mol Cell., 2019 Feb 7;73(3):601-610.
13. Wright AV#, Liu JJ#, Knott GJ, Doxzen KW, Nogales E, Doudna JA*. Structures of the CRISPR genome integration complex. Science, 2017 Sep 15;357(6356):1113-1118.
#: Co-first author; *: Corresponding author
● Contact info：
E-mail：junjiegogoliu(at)tsinghua.edu.cn (Please change (at) to @ when contacting)
Website：http://gogolab.life.tsinghua.edu.cn
Tel：+86-010-62770270 (lab) +86-010-62770276 (office)
Address： A401, Bio-Medical Building, School of Life Sciences, Tsinghua University, Beijing, 100084, China
