Jun-Jie (Gogo) Liu Ph.D.
Associate Professor
2007-2011 B.S., School of Life Sciences, Sichuan University
2011-2016 Ph.D., School of Life Sciences, Tsinghua University
2016-2018 Postdoctoral Fellow, MBIB Division, Lawrence Berkeley National Laboratory
2018-2020 Postdoctoral Fellow, Department of Molecular and Cell Biology, UC Berkeley
2018-2020 Life Science Research Fund Fellow Sponsored by Pfizer
2020-present Assistant professor, Associate professor, School of Life Sciences, Tsinghua University
2020-present PI, Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University
Beijing Frontier Research Center for Biological Structure, Tsinghua University
● Research Interest：
The Liu lab uses multidisciplinary approaches to study the following topics:
（1） Design and development of new gene-editing tools
（2） Mechanism study about RNA-involved nuclease machinery
● Selected Publications:
1. Liu ZX#, Zhang S#, Zhu HZ#, Chen ZH#, Yang Y#, Li LQ#, Lei Y, Liu Y, Li DY, Sun A, Li CP, Tan SQ, Wang GL, Shen JY, Jin S, Gao C, Liu JJ*. Hydrolytic endonucleolytic ribozyme (HYER) is programmable for sequence-specific DNA cleavage. Science, 2024 Feb 2; 383(6682): eadh4859.
2. Deng P#, Tan SQ#, Yang QY, Fu L, Wu Y, Zhu HZ, Sun L, Bao Z, Lin Y, Zhang QC, Wang H, Wang J*, Liu JJ*. Structural RNA components supervise the sequential DNA cleavage in R2 retrotransposon. Cell, 2023 Jun 22;186(13):2865-2879.
3. Li DY#, Li LQ#, Liu JJ*. Nucleases in gene-editing technologies: past and prologue. National Science Open, 2023 Jul 18;2(5): 20220067.
4．Hu Z#, Sun A#, Yang J, Naz G, Sun G, Li Z, Liu JJ*, Zhang S*, Zhang X*. Regulation of the CRISPR-Cas12a system by methylation and demethylation of guide RNA. Chemical Science, 2023,14(22):5945-5955.
5. Sun A#, Li CP#, Chen Z#, Zhang S#, Li DY, Yang Y, Li LQ, Zhao Y, Wang K, Li Z, Liu J, Liu S, Wang J*, Liu JJ*. The compact Casπ (Cas12l) ‘bracelet’ provides a unique structural platform for DNA manipulation. Cell Research, 2023 Mar;33(3), 229–244.
6. Tsuchida CA#, Zhang S#, Doost MS#, Zhao Y#, Wang J, O'Brien E, Fang H, Li CP, Li D, Hai ZY, Chuck J, Brötzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA*, Liu JJ*. Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity. Mol Cell., 2022 Mar 17;82(6):1199-1209.
7. Yang M, Sun R, Deng P, Yang Y, Wang W, Liu JJ*, Chen C*. Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search. Chem Sci., 2021 Aug 20;12(38):12776-12784.
8. Liu JJ*, Wang HW*. Cryo-Electron Microscopy of Endogenous Yeast Exosomes. Methods in Molecular Biology, 2020;2062:401-415. (Book Chapter)
9. Liu TY#, Liu JJ#, Aditham AJ, Nogales E, Doudna JA*. Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble. Nat Commun., 2019 Jul 5;10(1):3001.
10. Liu JJ#, Orlova N#, Oakes BL#, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E*, Doudna JA*. Author Correction: CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature, 2019 Apr;568(7752):E8-E10.
11. Jiang F#, Liu JJ#, Osuna BA#, Xu M, Berry JD, Rauch BJ, Nogales E, Bondy-Denomy J*, Doudna JA*. Temperature-Responsive Competitive Inhibition of CRISPR-Cas9. Mol Cell., 2019 Feb 7;73(3):601-610.
12. Wright AV#, Liu JJ#, Knott GJ, Doxzen KW, Nogales E, Doudna JA*. Structures of the CRISPR genome integration complex. Science, 2017 Sep 15;357(6356):1113-1118.
#: Co-first author; *: Corresponding author
● Contact info：
E-mail：junjiegogoliu(at)tsinghua.edu.cn (Please change (at) to @ when contacting)
Website：http://gogolab.life.tsinghua.edu.cn
Tel：+86-010-62770270 (lab) +86-010-62770276 (office)
Address： A401, Bio-Medical Building, School of Life Sciences, Tsinghua University, Beijing, 100084, China
