Chunlai CHEN Ph.D.
Associate Professor
1999-2003 Peking University B.Sc.
2003-2008 Peking University Ph.D.
2008-2015 University of Pennsylvania Postdoc
2015-2018 School of Life Sciences Tsinghua University Assistant Professor
2018-now School of Life Sciences Tsinghua University Associate Professor
Research Area:
1) Develop cutting-edge single molecule fluorescence methods.
2) Dynamics and molecular mechanisms of target searching of proteins on DNA.
3) Molecular mechanisms of CRISPR proteins in target searching and cleavage.
Selected publications
1. Yuan Zhang (#), Chen Yang (#), Sijia Peng, Jing Ling, Peng Chen, Yumiao Ma, Wenjuan Wang, Zhixing Chen (*), and Chunlai Chen (*), (2023) General strategy to improve the photon budget of thiol-conjugated cyanine dyes, Journal of the American Chemical Society, 145, 7, 4187-4198
2. Ruirui Sun, Yuqian Zhao, Wenjuan Wang, Jun-Jie Gogo Liu, and Chunlai Chen (*), (2023) Nonspecific interactions between Cas12a and dsDNA located downstream of the PAM mediate target search and assist AsCas12a for DNA cleavage, Chem Sci, 14, 3839-3851
3. Xiaolin Niu, Zhonghe Xu, Yufan Zhang, Xiaobing Zuo, Chunlai Chen (*) and Xianyang Fang (*), (2023) Structural and dynamic mechanisms for coupled folding and tRNA recognition of a translational T-box riboswitch, Nat Commun, 14(1), 7394
4. Jie Heng, Yunfei Hu, Guillermo Pérez-Hernández, Asuka Inoue, Jiawei Zhao, Xiuyan Ma, Xiaoou Sun, Kouki Kawakami, Tatsuya Ikuta, Jienv Ding, Yujie Yang, Lujia Zhang, Sijia Peng, Xiaogang Niu, Hongwei Li, Ramon Guixà-González, Changwen Jin, Peter W Hildebrand, Chunlai Chen (*), and Brian K. Kobilka (*), (2023) Function and dynamics of the intrinsically disordered carboxyl terminus of β2 adrenergic receptor, Nat Commun, 14(1), 2005
5. Jingfei Xu(#), Lingyun Zhao(#), Sijia Peng(#), Huiying Chu(#), Rui Liang, Meng Tian, Philip P. Connell, Guohui Li (*), Chunlai Chen (*) and Hong-Wei Wang (*), (2021) Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination, Nucleic Acids Research, 49, 13135-13149
6. Xiaolin Niu(#), Ruirui Sun(#), Zhifeng Chen, Yirong Yao, Xiaobing Zuo, Chunlai Chen (*) and Xianyang Fang (*), (2021) Pseudoknot length modulates the folding, conformational dynamics and robustness of Xrn1 resistance of flaviviral xrRNAs, Nat Commun, 12, 6417
7. Mengyi Yang, Ruirui Sun, Pujuan Deng, Yuzhuo Yang, Wenjuan Wang, Jun-Jie Gogo Liu (*) and Chunlai Chen (*), (2021) Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search, Chem Sci, 12, 12776-12784
8. Ming-Jie Han(#), Qing-tao He(#), Mengyi Yang(#), Chao Chen, Yirong Yao, Xiaohong Liu, Yuchuan Wang, Zhong-liang Zhu, Kong-kai Zhu, Changxiu Qu, Fan Yang, Cheng Hu, Xuzhen Guo, Dawei Zhang, Chunlai Chen (*), Jin-peng Sun (*) and Jiangyun Wang (*), (2021) Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and a Se-click reaction, Chem Sci, 12, 9114-9123
9. Sijia Peng (#), Weiping Li (#), Yirong Yao (#), Wenjing Xing, Pilong Li (*) and Chunlai Chen (*), (2020) Phase separation at the nanoscale quantified by dcFCCS, PNAS, 117(44):27124-27131
10. Sijia Peng (#), Xiaowei Wang (#), Lu Zhang (#)(*), Shanshan He, Xin Sheng Zhao (*), Xuhui Huang (*) and Chunlai Chen (*), (2020) Target search and recognition mechanisms of glycosylase AlkD revealed by scanning FRET-FCS and Markov State Models, PNAS, 117(36):21889-21895
Contact information
email :chunlai@tsinghua.edu.cn
