Yi XUE Ph.D.
Assistant Professor
1993-1997 B.S. Tsinghua University, Modern Applied Physics
1997-1999 Engineer Start Computer Company
2000-2003 M.S. Tsinghua University, Biological Science and Biotechnology
2004-2009 Ph.D. Purdue University, Chemistry
2010-2013 Postdoc Purdue University
2013-2016 Postdoc University of Michigan; Duke University
2016-2024 Assistant Professor Tsinghua University
2024-present Associate Professor Tsinghua University
Research interest
Our primary research interests are focused on characterizing structure and dynamics of non-coding RNAs and intrinsically disordered proteins using solution NMR spectroscopy and computational approaches such as MD simulations. We aim to develop novel NMR techniques, labeling methods, and computational tools to study three-dimensional structure and conformational switch of large non-coding RNAs and ribonucleoproteins, which pose a significant challenge to existing methods. We are also interested in reconstructing conformational motion of highly flexible biomolecules, as well as ensemble-based virtual drug screening.
Selected publications
1. Liu N, Mikhailovskii O, Skrynnikov NR* and Xue Y*. (2023). Simulating diffraction photographs based on molecular dynamics trajectories of a protein crystal: a new option to examine structure-solving strategies in protein crystallography. IUCrJ 10, 16-26
2. Luan X, Li X, Li Y, Su G, Yin W, Jiang Y, Xu N, Wang F, Cheng W, Jin Y, Zhang L, Xu HE*, Xue Y* and Zhang S*. (2022). Antiviral drug design based on structural insights into the N-terminal domain and C-terminal domain of the SARS-CoV-2 nucleocapsid protein. Science Bulletin 67, 2327-2335
3. He W, Li X, Xue H, Yang Y, Mencius J, Bai L, Zhang J, Xu J, Wu B, Xue Y* and Quan S*. (2022). Insights into the client protein release mechanism of the ATP-independent chaperone Spy. Nat. Commun. 13, 2818
4. Han G and Xue Y*. (2022). Rational design of hairpin RNA excited states reveals multi-step transitions. Nat. Commun. 13, 1523
5. Li W, Kou J, Qin J, Li L, Zhang Z, Pan Y, Xue Y* and Du W*. (2021). NADPH levels affect cellular epigenetic state by inhibiting HDAC3–Ncor complex. Nat. Metab. 3, 75-89
6. Cao J and Xue Y*. (2021). Characteristic chemical probing patterns of loop motifs improve prediction accuracy of RNA secondary structures. Nucleic Acids Res. 49, 4294-4307
7. Wang Y, Han G, Jiang X, Yuwen T and Xue Y*. (2021). Chemical shift prediction of RNA imino groups: application toward characterizing RNA excited states. Nat. Commun. 12, 1595
8. Song Y, Zhang Y, Pan Y, He J, Wang Y, Chen W, Guo J, Deng H, Xue Y*, Fang X* and Liang X*. (2020). The microtubule end-binding affinity of EB1 is enhanced by a dimeric organization that is susceptible to phosphorylation. J. Cell Sci. 133, jcs241216
Contact information
Email: yixue@mail.tsinghua.edu.cn
Phone: +86-10-62784766 (lab)
