王佳伟 博士

副教授

2001年／中国科学技术大学／学士

2005年／中国科学院物理研究所／博士

2005－2008年／美国阿岗国家实验室／博士后

2008－2011年／清华大学生命科学学院／高级工程师

2011－2017年／清华大学生命科学学院／助理教授

2017年-至今／清华大学生命科学学院／副教授

●  研究兴趣、领域：

本实验室主要利用X射线晶体学和冷冻电镜方法研究细菌磷酸转移酶系统（PTS）的膜蛋白结构。其中甘露糖转运复合物（Man-PTS）是PTS的一类重要成员，负责葡萄糖、甘露糖及多种糖类的摄取。此外， Man-PTS被报道为相关细菌素的作用靶点，其参与或介导了杀菌作用。本课题组已经解析出细菌素作用靶点---大肠杆菌来源的Man-PTS的结构。后续将继续通过晶体学和冷冻电镜等手段研究细菌素与Man-PTS作用后的杀菌机制，并为新型抗菌药物和食品添加剂的设计研发提供思路。本课题组同时探索结构解析过程中的困难问题，发展新的结构分析方法。

●  代表性论文：

1. Li R, Duan J, Zhou Y, Wang J*. Structural basis of the mechanisms of action and immunity of lactococcin A, a class IId bacteriocin. Appl Environ Microbiol. 2023; 89:e00066-23.

2. Zhu L, Zeng J, Wang J*. Structural basis of the immunity mechanisms of pediocin-like bacteriocins. Appl Environ Microbiol. 2022; 88(13):e0048122.

3. Wang C, Zeng J*, Wang J*. Structure basis of bacteriophage lambda capsid maturation. Structure, 2022; S0969-2126(21)00461-5.

4. Zhu L, Zeng J, Wang C, Wang J*. Structural basis of pore formation in the mannose phosphotransferase system by pediocin PA-1. Appl Environ Microbiol. 2022; 88(3):e0199221.

5. Huang K, Zeng J, Liu X, Jiang T*, Wang J*. Structure of the mannose phosphotransferase system (man-PTS) complexed with microcin E492, a pore-forming bacteriocin. Cell Discovery, 2021;7(1):20.

6. Li L, Dai S, Gao GF*, Wang J*. Lattice-translocation defects in specific crystals of the catalytic head domain of influenza neuraminidase. Acta Crystallogr D Struct Biol. 2020;76:1057-1064 (Cover).

7. Su R*, Zhang S, Wang J*. Automatically generating mask for mapping out the density map in cryoEM single-particle reconstruction. Progress in Biochemistry and Biophysics, 2019; 46:1020-1030.

8. Liu X, Zeng J, Huang K, Wang J*. Structure of the mannose transporter of the bacterial phosphotransferase system. Cell Research 2019;29(8):680-682.

9. Qi X, Schmiege P, Coutavas E, Wang J*, Li X*. Structures of human Patched and its complex with native palmitoylated sonic hedgehog. Nature. 2018;7716:128-132.

10. Liu X, Wang J*, Sun L*. Structure of the hypersomolality-gated calcium-permeable channel OSCA1.2. Nat Commun. 2018; 9(1):5060.

11. Zhou N, Wang H*, Wang J*. EMBuilder: A Template Matching-based Automatic Model-building Program for High-resolution Cryo-Electron Microscopy Maps. Sci Rep. 2017;7(1):2664.

12. Wang J*, Chai J, Wang H. Structure of the mouse Toll-like receptor 13 ectodomain in complex with a conserved sequence from bacterial 23S ribosomal RNA. FEBS J. 2016;283(9):1631-5 (Cover).

13. Wang J, Wang W, Song W, Han Z, Zhang H, Chai J*, Wang H*, Wang J*. An improved method for phasing crystal structures with low non-crystallographic symmetry using cryo-electron microscopy data. Protein Cell. 2015;6(12):919-23.

14. Luo P, Dai S, Zeng J, Duan J, Shi H, Wang J*. Inward-facing conformation of L-ascorbate transporter suggests an elevator mechanism. Cell Discovery, 2018;4:35.

Luo P, Yu X, Wang W, Fan S, Li X*, Wang J*. Crystal structure of a phosphorylation-coupled vitamin C transporter. Nat Struct Mol Biol. 2015;22(3):238-41 (Cover).

●  联系方式：

电话：+86-10-62782124

传真：+86-10-62782124

邮箱：jwwang@tsinghua.edu.cn

通讯地址：清华大学生物医学馆A317室