陈春来 博士

副教授

1999-2003      北京大学化学与分子工程学院        学士

2003-2008     北京大学化学与分子工程学院         博士

2008-2015      University of Pennsylvania             博士后

2015-2018      清华大学生命科学学院                   助理教授

2018-至今       清华大学生命科学学院                   副教授

研究兴趣、领域

1) 发展新型单分子荧光技术；

2) 蛋白在DNA上的靶向搜寻机制的研究；

3) CRISPR系列蛋白靶向和剪切机制的研究。

代表性论文

1.  Yu Miao (#), Yuxiao Cheng (#), Yushi Xia, Yongzhen Hei, Wenjuan Wang, Qionghai Dai (*), Jinli Suo (*), Chunlai Chen (*), (2025) Supervised multi-frame dual-channel denoising enables long-term single-molecule FRET under extremely low photon budget, Nat Commun, 16(1), 74

2.  Sudheer K. Cheppali (#), Chang Li (#), Wenjing Xing (#), Ruirui Sun, Mengyi Yang, Yi Xue, Si-Yao Lu, Jun Yao, Shan Sun (*), Chunlai Chen (*), Sen-Fang Sui (*), (2024) Single-molecule two- and three-colour FRET studies reveal a transition state in SNARE disassembly by NSF, Nat Commun, 16(1), 250

3.  Jiawei Zhao (#), Matthias Elgeti (#)(*), Evan S. O’Brien, Cecília P. Sár, Amal EI Daibani, Jie Heng, Xiaoou Sun, Elizabeth White, Tao Che, Wayne L. Hubbell, Brian K. Kobilka (*), and Chunlai Chen (*), (2024) Ligand efficacy modulates conformational dynamics of the µ-opioid receptor, Nature, 629, 474–480

4.  Shouyue Zhang (#), Ao Sun (#), Jing-Mei Qian (#), Shuo Lin (#), Wenjing Xing, Yun Yang, Han-Zhou Zhu, Xin-Yi Zhou, Yan-Shuo Guo, Yun Liu, Yu Meng, Shu-Lin Jin, Cheng-Ping Li, Zhaofu Li, Shuai Jin, Jian-Hua Wang, Meng-Qiu Dong, Caixia Gao, Chunlai Chen (*), Yang Bai (*), Jun-Jie Gogo Liu (*), (2024) Pro-CRISPR PcrIIC1 associated Cas9 system for enhanced bacterial immunity, Nature, 630, 484-492

5.  Wenjing Xing (#), Danyuan Li (#), Wenjuan Wang, Jun-Jie Gogo Liu (*), Chunlai Chen (*), (2024) Conformational dynamics of CasX (Cas12e) in mediating DNA cleavage revealed by single-molecule FRET, Nucleic Acids Research, 52, 9014-9027

6.  Shuqi Zhou, Yu Miao, Haoren Qiu, Yuan Yao, Wenjuan Wang, Chunlai Chen (*), (2024) Deep learning based local feature classification to automatically identify single molecule fluorescence events, Communications Biology, 7, 1404

7.  Danyuan Li (#), Shouyue Zhang (#), Shuo Lin (#), Wenjing Xing (#), Yun Yang, Fengxia Zhu, Dingding Su (*), Chunlai Chen (*), Jun-Jie Gogo Liu (*), (2024) Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavage, Nat Commun, 15, 10727

8.  Yuan Zhang (#), Chen Yang (#), Sijia Peng, Jing Ling, Peng Chen, Yumiao Ma, Wenjuan Wang, Zhixing Chen (*), and Chunlai Chen (*), (2023) General strategy to improve the photon budget of thiol-conjugated cyanine dyes, Journal of the American Chemical Society, 145, 7, 4187-4198

9.  Ruirui Sun, Yuqian Zhao, Wenjuan Wang, Jun-Jie Gogo Liu, and Chunlai Chen (*), (2023) Nonspecific interactions between Cas12a and dsDNA located downstream of the PAM mediate target search and assist AsCas12a for DNA cleavage, Chem Sci, 14, 3839-3851

10. Xiaolin Niu, Zhonghe Xu, Yufan Zhang, Xiaobing Zuo, Chunlai Chen (*) and Xianyang Fang (*), (2023) Structural and dynamic mechanisms for coupled folding and tRNA recognition of a translational T-box riboswitch, Nat Commun, 14(1), 7394

联系方式

邮箱：chunlai@tsinghua.edu.cn