陈春来 博士

副教授


1999-2003    学士     北京大学化学与分子工程学院

2003-2008    博士      北京大学化学与分子工程学院

2008-2015    博士后/研究员     University of Pennsylvania

2015-至今     研究员        清华大学生命科学学院,清华-北大生命科学联合中心(CLS)

 

研究兴趣、领域


1) 发展新型单分子荧光技术;

2) 蛋白在DNA上的靶向搜寻机制的研究;

3) CRISPR系列蛋白靶向和剪切机制的研究。

 

代表性论文


1. Sijia Peng (#), Weiping Li (#), Yirong Yao (#), Wenjing Xing, Pilong Li (*),and Chunlai Chen (*), Phase separation at the nanoscale quantified by dcFCCS, PNAS, 117(44):27124-27131

2. Sijia Peng (#), Xiaowei Wang (#), Lu Zhang (#)(*), Shanshan He, Xin Sheng Zhao (*), Xuhui Huang (*), and Chunlai Chen (*),Target search and recognition mechanisms of glycosylase AlkD revealed by scanning FRET-FCS and Markov State Models, PNAS, 117(36):21889-21895

3. Man Pan (#), Qingyun Zheng (#), Shan Ding (#), Lujia Zhang (#), Qian Qu, Tian Wang, Danning Hong, Yunjing Ren, Lujun Liang, Chunlai Chen (*), Ziqing Mei (*), and Lei Liu (*), (2019) Chemical Protein Synthesis-Enabled Mechanistic Studies on the Molecular Recognition of K27-linked Ubiquitin Chains, Angew. Chem. Int. Ed, 58(9):2627-2631

4. Bo Wu, Haibo Zhang, Ruirui Sun, Sijia Peng, Barry S Cooperman, Yale E Goldman (*), and Chunlai Chen (*), (2018) Translocation kinetics and structural dynamics of ribosomes are modulated by the conformational plasticity of downstream pseudoknots, Nucleic Acids Research, 46(18): 9736-9748

5. Sijia Peng, Wenjuan Wang, and Chunlai Chen(*), (2018) Surface Transient Binding-Based Fluorescence Correlation Spectroscopy (STB-FCS), a Simple and Easy-to-Implement Method to Extend the Upper Limit of the Time Window to Seconds, J. Phys. Chem. B, 122(18): 4844-4850

6. Mengyi Yang(#), Sijia Peng(#), Ruirui Sun, Jingdi Lin, Nan Wang, and Chunlai Chen(*), (2018) The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET, Cell Reports, 22(2): 372-382

7. Sijia Peng, Mengyi Yang, Rui Ning Sun, Yang Liu, Wenjuan Wang, Qiaoran Xi, Haipeng Gong, and Chunlai Chen(*), (2018) Mechanism of actions of Oncocin, a proline-rich antimicrobial peptide, in early elongation revealed by single-molecule FRET, Protein & Cell, 9(10): 890-895

8. Sijia Peng, Ruirui Sun, Wenjuan Wang and Chunlai Chen(*), (2017) Single-Molecule Photoactivation FRET: A General and Easy-to-Implement Approach to Break the Concentration Barrier, Angew. Chem. Int. Ed, 56(24):6882-6885

 

联系方式


邮箱:chunlai@tsinghua.edu.cn