李赛 博士

副教授


2018年-至今: 清华大学生命学院 研究员
2018年-至今: 清华-北大生命联合中心 研究员
2018年-至今: 结构生物学高精尖创新中心 研究员
2017-2018年: 英国牛津大学粒子成像中心 Senior Research Associate
2013-2017年: 英国牛津大学粒子成像中心 博士后
2009-2012年: 德国哥廷根大学生物物理系 博士
2006-2008年: 德国斯图加特大学物理系 硕士
2002-2006年: 武汉大学应用物理系 学士

 

●  研究兴趣、领域:

1.     高分辨冷冻电镜断层成像(cryo-ET)方法。高分辨cryo-ET是通过收集三维断层图像,再使用子断层图像平均法进行结构解析的前沿结构生物学方法。Cryo-ET擅长对柔性巨型分子机器、细菌、细胞进行成像和蛋白结构解析,获得具生理意义的原位结构,是结构生物学的下一个突破。    
 

2. 新发囊膜病毒的组装及在细胞中复制活动的结构与功能。由于形态不均一,囊膜病毒的原位结构解析颇具挑战。我们使用cryo-ET解析全病毒结构,并在细胞中直接观测病毒生命活动的原位结构,希望揭示囊膜病毒的组装机制,并揭秘它们在细胞中的生命活动。最终目的是找到病毒的弱点,并为抗体、疫苗设计提供新思路。

●  代表性论文:


1. Sai Li: Cryo-electron tomography of enveloped viruses. Trends in Biochemical Sciences, 09/2021; DOI:10.1016/j.tibs.2021.08.005

2. Joel D. Allen, Himanshi Chawla, Firdaus Samsudin, Lorena Zuzic, Aishwary Tukaram Shivgan, Yasunori Watanabe, Wan-ting He, Sean Callaghan, Ge Song, Peter Yong, Philip J. M. Brouwer, Yutong Song, Yongfei Cai, Helen Duyvesteyn, Tomas Malinauskas, Joeri Kint, Paco Pino, Maria J. Wurm, Martin Frank, Bing Chen, David I. Stuart, Rogier W. Sanders, Raiees Andrabi, Dennis R. Burton, Sai Li, Peter J. Bond, and Max Crispin: Site-Specific Steric Control of SARS-CoV 2 Spike Glycosylation. Biochemistry, 07/2021; DOI: 10.1021/acs.biochem.1c00279

封面文章

3. Hangping Yao*, Yutong Song*, Yong Chen*, Nanping Wu*, Jialu Xu*, Chujie Sun, Jiaxing Zhang, Tianhao Weng, Zheyuan Zhang, Zhigang Wu, Linfang Cheng, Danrong Shi, Xiangyun Lu, Jianlin Lei, Max Crispin, Yigong Shi, Lanjuan Li#, and Sai Li#: Molecular architecture of the SARS-CoV-2 virus. Cell, 2020; DOI:10.1016/J.CELL.2020.09.018

ESI高被引论文; F1000推荐

入选2020年“中国高校十大科技进展”

4. Alexandra Serris, Robert Stass, Eduardo A. Bignon, Nicolas A. Muena, Jean-Claude Manuguerra, Rohit K. Jangra, Sai Li, Kartik Chandran, Nicole Tischler, Juha T. Huiskonen, Felix A. Rey, and Pablo Guardado-Calvo: Description of the Hantavirus Surface Glycoprotein Lattice and Its Membrane Fusion Control Mechanism. Cell, 2020; DOI:10.1016/J.CELL.2020.08.023

5. Ying Liu*, Yutong Song*, Siyu Zhang, Min Diao, Shanjin Huang, Sai Li, and Xu Tan: PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteins. Cell Discovery, 12/2020; DOI:10.1038/s41421-020-0184-9

6. Yanqing Zhang*, Sai Li*, Xuechen Zhu*, Gaoxingyu Huang*, Chao Zeng*, Qifan Wang, Kunpeng Wang, Qiang Zhou, Wusheng Zhang, Guangwen Yang, Minhao Liu, Qinghua Tao, Jianlin Lei, and Yigong Shi: Molecular architecture of the luminal ring of the Xenopus laevis nuclear pore complex. Cell Research, 05/2020; DOI:10.1038/s41422-020-0320-y

F1000推荐;封面文章

7. Xiaoling Wu*, Hua Yue*, Yuanyu Zhang*, Xiaoyong Gao, Xiaoyang Li, Licheng Wang, Yufei Cao, Miao Hou, Haixia An, Lin Zhang#, Sai Li#, Jingyuan Ma, He Lin, Yanan Fu, Hongkai Gu, Wenyong Lou, Wei Wei#, Richard N. Zare, and Jun Ge#: Packaging and delivering enzymes by amorphous metal-organic frameworks. Nature Communications, 11/2019; 10(1)., DOI:10.1038/s41467-019-13153-x

8. Kamel El Omari, Sai Li, Abhay Kotecha, Thomas S Walter, Eduardo Andres Bignon, Karl Harlos, Pentti Somerharju, Felix De Haas, Daniel K. Clare, Mika Molin, Felipe Hurtado, Mengqiu Li, Jonathan M. Grimes, Dennis H. Bamford, Nicole D. Tischler, Juha T. Huiskonen, David I. Stuart, Elina Roine: The structure of a prokaryotic viral envelope protein expands the landscape of membrane fusion proteins. Nature Communications, 02/2019; 10(1)., DOI:10.1038/s41467-019-08728-7

9. Steinar Halldorsson*, Sai Li*, Mengqiu Li, Karl Harlos, Thomas A. Bowden, Juha T. Huiskonen: Shielding and activation of a viral membrane fusion protein. Nature Communications, 01/2018; 9(349)., DOI:10.1038/s41467-017-02789-2

10. Sai Li, Ilona Rissanen, Antra Zeltina, Jussi Hepojoki, Jayna Raghwani, Karl Harlos, Oliver G. Pybus, Juha T. Huiskonen, Thomas A. Bowden: A Molecular-Level Account of the Antigenic Hantaviral Surface. Cell Reports, 06/2016; 16(1):278., DOI:10.1016/j.celrep.2016.06.039

11. Sai Li, Zhaoyang Sun, Rhys Pryce, Marie-Laure Parsy, Sarah K Fehling, Katrin Schlie, C Alistair Siebert, Wolfgang Garten, Thomas A Bowden, Thomas Strecker, Juha T Huiskonen: Acidic pH-Induced Conformations and LAMP1 Binding of the Lassa Virus Glycoprotein Spike. PLoS Pathogens, 02/2016; 12(2)., DOI:10.1371/journal.ppat.1005418

12. Sai Li*, Christian Sieben*, Kai Ludwig, Chris T Höfer, Salvatore Chiantia, Andreas Herrmann, Frederic Eghiaian, Iwan A.T. Schaap: pH-Controlled Two-Step Uncoating of Influenza Virus. Biophysical Journal, 04/2014; 106(7):1447-56., DOI:10.1016/j.bpj.2014.02.018

13. Sai Li, Frederic Eghiaian, Christian Sieben, Andreas Herrmann, Iwan A.T. Schaap: Bending and Puncturing the Influenza Lipid Envelope. Biophysical Journal, 02/2011; 100(3):637-45., DOI:10.1016/j.bpj.2010.12.3701

14. Felix Hoos,Sai Li, Todd Meyrath, Bernd Braun, and Harald Giessen: Thermal lensing in an end-pumped Yb:KGW slab laser with high power single emitter diodes.Optics Express, 04/2008; 16(9):6041-9., DOI:10.1364/OE.16.006041

* Equal contribution. # Corresponding Author.

 

●  联系方式:


网页:http://www.lisailab.com

电话:+86-10-62792357

邮箱:sai@mail.tsinghua.edu.cn

通讯地址:清华大学生物医学馆A318