生物成像技术讲座通知

2018-04-29 16:07:53

生命学科校级平台及结构生物学高精尖创新中心邀请北京大学神经科学研究所张勇博士于5月8日下午2点在生物新馆143做关于神经突触可塑性在体成像讲座。 张勇博士现为北京大学神经科学研究所及麦戈文脑研究所研究员,曾获得2006年国家优秀自费留学生奖学金,2015年约翰霍普金斯大学杰出青年科学家奖。张勇博士的主要研究方向是阐述在健康和疾病状态下神经可塑性以及学习和记忆的分子机制。具体运用双光子活体成像技术研究神经元表面AMPA型谷氨酸受体动态变化,神经元活性(运用遗传编码的钙离子指示剂,如GCaMP6),以及神经元内多种信号通路的活性(运用FRET或者单色信号通路生物传感器,Biosensors)对动物行为以及学习和记忆对影响。

讲座时间:2018年5月8日  14:00-15:00

地点:生物新馆143

报名方式:张泽惠 (zhangzehui@biomed.tsinghua.edu.cn)

邮件注明:姓名、课题组、手机号、学号、院系

请于5月7日下午17:00前报名

有兴趣与张勇博士面谈的老师请联系王亚林 (wyl2017@mail.tsinghua.edu.cn)

Imaging synaptic plasticity in vivo

Yong Zhang

Neuroscience Research Institute, Peking University

Regulation of AMPA receptor (AMPAR) membrane trafficking is critical for synaptic plasticity, as well as for learning and memory. However, the mechanisms of AMPAR trafficking in vivo remain elusive. Using in vivo two-photon microscopy in the mouse somatosensory barrel cortex, we found that acute whisker stimulation led to a significant increase in the intensity of surface AMPAR GluA1 subunit (sGluA1) in both spines and dendritic shafts and a small increase in spine size relative to prestimulation values. The increase in spine sGluA1 intensity evoked by whisker stimulation was NMDA receptor dependent and long lasting, similar to major forms of synaptic plasticity in the brain. We were able to observe experience-dependent AMPAR trafficking in real time and characterize, in vivo, a major form of synaptic plasticity in the brain.