闫创业 博士

研究员

2005-2009年/清华大学/学士                        

2009-2015年/清华大学/博士 

2015-2019年/清华大学/博士后        

2019-至今   /清华大学生命科学学院/研究员

 

● 研究兴趣、领域:

1.     X-射线晶体学以及冷冻电镜方法的开发及应用。

2.     重要膜蛋白的结构生物学研究以及细胞分裂过程中亚显微结构的研究。

3.     真核细胞中剪接体的结构和功能以及前体信使RNA剪接机制的研究。

 

● 代表性论文:

[1] Yan, C.*, Wan, R.* & Shi, Y.. Molecular Mechanisms of pre-mRNA Splicing through Structural Biology of the Spliceosome. Cold Spring Harb Perspect Biol 11(2019).

[2] Zhang, X., Zhan, X., Yan, C.*#, Zhang, W., Lei, J. & Shi, Y. Structures of the human spliceosomes before and after release of the ligated exon. Cell Res 29, 274-285 (2019).

[3] Zhan, X., Yan, C.*#, Zhang, X., Lei, J. & Shi, Y. Structures of the human pre-catalytic spliceosome and its precursor spliceosome. Cell Res 28, 1129-1140 (2018).

[4] Bai, R.*, Wan, R.*, Yan, C.*, Lei, J. & Shi, Y. (2018). Structures of the fully assembled Saccharomyces cerevisiae spliceosome before activation. Science, doi:10.1126/science.aau0325.

[5] Zhang, X.*, Yan, C.*#, Zhan, X.*, Li, L.*, Lei, J., and Shi, Y. (2018). Structure of the human activated spliceosome in three conformational states. Cell research, doi:10.1038/cr.2018.14.

[6] Zhan, X.*, Yan, C.*#, Zhang, X.*, Lei, J. & Shi, Y. (2018). Structure of a human catalytic step I spliceosome. Science, doi:10.1126/science.aar6401.

[7] Zhang, X.*, Yan, C.*#, Hang, J.*, Finci, L.I., Lei, J., and Shi, Y. An Atomic Structure of the Human Spliceosome. Cell 169, 918-929 e914, doi:10.1016/j.cell.2017.04.033 (2017).

[8] Yan, C.*, Wan, R.*, Bai, R.*, Huang, G. & Shi, Y. Structure of a yeast step II catalytically activated spliceosome. Science 355, 149-155, doi:10.1126/science.aak9979 (2017).

[9] Wan, R.*, Yan, C.*, Bai, R.*, Lei, J. & Shi, Y. (2017). Structure of an Intron Lariat Spliceosome from Saccharomyces cerevisiae. Cell 171, 120-132 e112.

[10] Bai, R.*, Yan, C.*, Wan, R.*, Lei, J. & Shi, Y. (2017). Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae. Cell 171, 1589-1598.

[11] Yan, C.*, Wan, R.*, Bai, R.*, Huang, G. & Shi, Y. (2016). Structure of a yeast activated spliceosome at 3.5 A resolution. Science 353, 904-911,

[12] Wan, R.*, Yan, C.*, Bai, R.*, Wang, L., Huang, M., Wong, C.C., and Shi, Y. (2016). The 3.8 A structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysis. Science 351, 466-475, doi:10.1126/science.aad6466 (2016).

[13] Wan, R.*, Yan, C.*, Bai, R.*, Huang, G.* & Shi, Y. (2016) Structure of a yeast catalytic step I spliceosome at 3.4 A resolution. Science 353, 895-904, doi:10.1126/science.aag2235 (2016).

[14] Yan, C.*, Hang, J.*, Wan, R.*, Huang, M., Wong, C.C., and Shi, Y. (2015). Structure of a yeast spliceosome at 3.6-angstrom resolution. Science 349, 1182-1191, doi:10.1126/science.aac7629 (2015).

[15] Hang, J.*, Wan, R.*, Yan, C.* & Shi, Y. (2015). Structural basis of pre-mRNA splicing. Science 349, 1191-1198, doi:10.1126/science.aac8159.

[16] Yan, Z.*, Bai, X.*, Yan, C.*, Wu, J., Li, Z., Xie, T., Peng, W., Yin, C., Li, X., Scheres, S.H.W., et al. (2015). Structure of the rabbit ryanodine receptor RyR1 at near-atomic resolution. Nature 517, 50-55.

[17] Bai, X.C.*, Yan, C.*, Yang, G.*, Lu, P., Ma, D., Sun, L., Zhou, R., Scheres, S.H.W., and Shi, Y. (2015). An atomic structure of human gamma-secretase. Nature 525, 212-217.

[18] Xie, T.*, Yan, C.*, Zhou, R., Zhao, Y., Sun, L., Yang, G., Lu, P., Ma, D., and Shi, Y. (2014). Crystal structure of the gamma-secretase component nicastrin. Proc Natl Acad Sci USA 111, 13349-13354.

[19] Deng D.*, Yan C.*, Pan X.*, Mahfouz M., Wang J., Zhu J.K., Shi Y., Yan N. (2012a) Structural basis for sequence-specific recognition of DNA by TAL effectors. Science 335: 720-723.

*: Co-first author; #: Corresponding authors

 

● 联系方式:

邮箱:yancy2019@mail.tsinghua.edu.cn 

通讯地址:北京市清华大学生命科学学院,闫创业实验室 100084