黄善金 博士

研究员,博导

1996年 原浙江农业大学农学专业 学士

2001年 中国农业大学生物学院植物学专业 博士

2001年~2006年 美国普渡大学生物系 博士后

2006年~2016年 中国科学院植物研究所 研究员

2014年~至今 清华大学生命科学学院 研究员

 

● 研究兴趣、领域:

综合分子遗传学、生物化学和细胞生物学等手段,利用花粉管为主要细胞学系统研究。

1)细胞骨架的结构与功能关系

2)细胞极性生长调控机制。

 

● 代表性论文:

1. Lan Y, Liu X, Fu Y* and Huang S*. Arabidopsis Class I Formins Control Membrane-Originated Actin Polymerization at Pollen Tube Tips. PLOS Genet. 2018, 14(11): e1007789

2. Diao M, Ren S, Wang Q, Qian L, Shen J, Liu Y and Huang S*. Arabidopsis Formin 2 Regulates Cell-to-Cell Trafficking by Capping and Stabilizing Actin Filaments at Plasmodesmata. eLife 2018;7:e36316

3. Diao M, Qu X and Huang S* . Calcium imaging in Arabidopsis pollen cells using G-CaMP5. J Integr Plant Biol. 2018;60(9):897-906

4. Jiang Y, Wang J, Xie Y, Chen N and Huang S* . Arabidopsis ADF10 Shapes the Organization of Apical Actin Filaments via Promoting their Turnover and Ordering in Pollen Tubes. J Cell Sci. 2017, 130, 3988-4001

5. Qu X, Zhang R, Zhang M, Diao M, Xue Y and Huang S* . Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning. Mol Plant. 2017, 10, 930–947

6. Chang M, Li Z and Huang S*. Monomeric G-actin is uniformly distributed in pollen tubes and is rapidly redistributed via cytoplasmic streaming during pollen tube growth. Plant J., 2017, 92(3): 509-519

7. Jiang Y, Zhang M and Huang S*. Analysis of Actin-based Intracellular Trafficking in Pollen Tubes. Methods Mol Biol., 2017, 1662:125-136

8. Yang Y, Zhang Y, Li W, Jiang Y, Zhu Z, Hu H, Li W, Wu J, Wang Z, Dong M, Huang S and Ou G. Spectraplakin Induces Positive Feedback between Fusogen and the Actin Cytoskeleton to Promote Cell-Cell Fusion. Dev Cell, 2017, 10; 41(1):107-120

9. Zhang R, Chang M, Zhang M, Wu Y, Qu X and Huang S*. The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins. J Biol Chem., 2016, 9;291(34):17881-96.

10. Zhang M, Zhang R, Qu X and Huang S* . Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube. J Exp Bot., 2016, 67(11):3407-17

11. Zhu Z, Chai Y, Jiang Y, Li W, Hu H, Li W, Wu J, Wang Z, Huang S and Ou G. Functional Coordination of WAVE and WASP in C. elegans Neuroblast Migration. Dev Cell, 2016, 24;39(2):224-238

12. Cao T, Sun L, Jiang Y, Huang S, Wang J and Chen Z. Crystal structure of a nuclear actin ternary complex. Proc Natl Acad Sci U S A., 2016, 113(32):8985-90

13. Zhao S, Jiang Y, Zhao Y, Huang S, Yuan M, Zhao Y and Guo Y. CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing Factor. Plant Cell, 2016, 28(6):1422-39

14. Liu X, Qu X, Jiang Y, Chang M, Zhang R, Wu Y, Fu Y and Huang S*. Profilin regulates apical actin polymerization to control polarized pollen tube growth. Mol Plant, 2015, 8(12):1694-709 (cover story)

15. Chang M and Huang S* . Arabidopsis ACT11 modifies actin turnover to promote pollen germination and maintain the normal rate of tube growth. Plant J. , 2015, 83 (3):515-527

16. Wu S, Xie Y, Zhang J, Ren Y, Zhang X, Wang J, Guo X, Wu F, Sheng P, Wang J, Wu C, Wang H, Huang S* and Wan J*. VLN2 regulates plant architecture by affecting microfilament dynamics and polar auxin transport in rice. Plant Cell, 2015, 27(10):2829-2845

17. Tian D, Diao M, Jiang Y, Sun L, Zhang Y, Chen Z, Huang S and Ou G. Anillin regulates neuronal migration and neurite growth by linking RhoG to the actin cytoskeleton. Curr. Biol. , 2015, 4;25(9):1135-45

18. Zhou Z, Shi H, Chen B, Zhang R, Huang S and Fu Y. Arabidopsis RIC1 severs actin filaments at the apex to regulate pollen tube growth. Plant Cell, 2015, 27(4):1140-61

19. Deng Z, Liu L, Li T, Yan S, Kuang B, Huang S, Yan C and Wang T. OsKinesin-13A is an active microtubule depolymerase involved in glume length regulation via affecting cell elongation. 2015, Sci. Rep., 5: 9457

20. Qu X, Jiang Y, Chang M, Liu X, Zhang R and Huang S*. Organization and regulation of the actin cytoskeleton in the pollen tube. Front. Plant Sci. , 2015, 8;5:786

21. Huang S* , Qu X and Zhang R. Plant villins: versatile actin regulatory proteins. J Integr. Plant Biol. , 2015, 57:40-49

22. Li X, Li J, Wang W, Chen N, Ma T, Xi Y, Zhang X, Lin H, Bai Y, Huang S* and Chen Y*. ARP2/3 complex-mediated actin dynamics is required for hydrogen peroxide-induced stomatal closure in Arabidopsis. Plant Cell Environ., 2014,37:1548-60

23. Wang Q and Huang S* . Visualization of microtubules organization and dynamics in living Arabidopsis embryonic cells. Mol. Plant, 2014;7(8):1397-401

24. Zheng Y, Xie Y, Jiang Y, Qu X and Huang S*. Arabidopsis actin depolymerizing factor7 severs actin filaments and regulates actin cable turnover to promote normal pollen tube growth. Plant Cell, 2013, 25:3405-3423

25. Qu X, Zhang H, Xie Y, Wang J, Chen N and Huang S*. Arabidopsis villins promote actin turnover at pollen tube tips and facilitate the construction of actin collars. Plant Cell, 2013, 25:1803-17

26. Shi M, Xie Y, Zheng Y, Wang J, Su Y, Yang Q and Huang S*. Oryza sativa actin-interacting protein 1 is required for rice growth via promoting actin turnover. Plant J. , 2013, 73, 747-760

27. Zhao Y, Pan Z, Zhang Y, Qu X, Yang Y, Zhang Y, Jiang X, Huang S, Yuan M, Schumaker K and Guo Y. The Arp2/3 complex regulates mitochondrial-associated calcium signaling during salt stress in Arabidopsis. Plant Cell, 2013, 25, 4544-4599

28. Zheng Y, Xin H, Lin J, Liu CM and Huang S*. An Arabidopsis class II Formin, AtFH19, nucleates actin assembly, binds to the barbed end of actin filaments and antagonizes the effect of AtFH1 on actin dynamics. J Integr. Plant Biol., 2012, 54(10): 800-813

29. Bao C, Wang J, Zhang R, Zhang B, Zhang H, Zhou Y and Huang S*. Arabidopsis VILLIN2 and VILLIN3 act redundantly in sclerenchyma development via bundling of actin filaments. Plant J. , 2012,71: 962-975

30. Li J, Henty JL, Huang S, Wang X, Blanchoin L and Staiger CJ. 2012. Capping protein modulates the dynamic behavior of actin filaments in response to phosphatidic acid in Arabidopsis. Plant Cell, 2012, 24, 3742-3754

31. Yang W, Ren S, Zhang X, Gao M, Ye S, Qi Y, Zheng Y, Wang J, Zeng L, Qun Li, Huang S*, and He Z*. BENT UPPERMOST INTERNODE1 encodes the class II formin FH5 crucial for actin organization and rice development. Plant Cell, 2011, 23:661-80

32. Zhao Y, Zhao S, Mao T, Qu X, Cao W, Zhang L, Zhang W, He L, Li S, Ren S, Zhao J, Zhu G, Huang S, Ye K, Yuan M and Guo Y. The plant-specific actin binding protein SCAB1 stabilizes actin filaments and regulates stomatal movement in Arabidopsis. Plant Cell, 2011, 23: 2314-2330

33. Wu Y, Yan J, Zhang R, Qu X, Ren S, Chen N and Huang S* . Arabidopsis FIMBRIN5, an actin bundling factor, is required for pollen germination and pollen tube growth. Plant Cell, 2010, 22: 3745-3763

34. Zhang H, Qu X, Bao C, Khurana P, Wang Q, Xie Y, Zheng Y, Chen N, Blanchoin L, Staiger C.J and Huang S* . Arabidopsis VILLIN5, an actin filament bundling and severing protein, is required for normal pollen tube growth. Plant Cell, 2010, 22: 2749-2767

35. Zhu Y, Wang Y, Li R, Song X, Wang Q, Huang S, Jin J, Liu C and Lin J. Analysis of interactions among the CLAVATA3 receptors reveals a direct interaction between CLAVATA2 and CORY in Arabidopsis. Plant J. , 2010, 61: 223-233

36. Wang J, Wang H, Zhao P, Han L, Jiao G, Zheng Y, Huang S and Xia G. Overexpression of a profilin (GhPFN2) promotes the progression of developmental phases in cotton fibers. Plant Cell Physiol. , 2010, 51(8): 1276-1290

37. Khurana P, Henty JL, Huang S, Staiger AM, Blanchoin L and Staiger CJ. Arabidopsis VILLIN1 and VILLIN3 have overlapping and distinct activities in actin bundle formation and turnover. Plant Cell, 2010, 22: 2727-2748

38. Ye J, Zheng Y, Yan A, Chen N, Wang Z, Huang S* and Yang Z*. Arabidopsis formin3 directs the formation of actin cables and polarized growth in pollen tubes. Plant Cell, 2009, 21:3868–3884

39. Chen N, Qu X, Wu Y and Huang S*. Regulation of actin dynamics in pollen tubes: control of actin polymer level. J Integr. Plant Biol., 2009, 51(8): 740–750

40. Zhang C, Mallery EL, Schlueter J, Huang S, Fan Y, Brankle S, Staiger CJ and Szymanski DB. Arabidopsis SCARs function interchangeably to meet actin-related protein 2/3 activation thresholds during morphogenesis. Plant Cell, 2008, 20(4): 995-1011

41. Yang G, Gao P, Zhang H, Huang S and Zheng Z-L. A mutation in MRH2 kinesin enhances the root hair tip growth defect caused by constitutively activated ROP2 small GTPase in Arabidopsis. PLoS ONE, 2007, 2(10): e1074

42. Michelot A, Derivery E, Paterski-Boujemaa R, Guerin C, Huang S, Parcy F, Staiger CJ and Blanchoin, L. A novel mechanism for the formation of actin-filament bundles by a nonprocessive formin. Curr. Biol., 2006, 16, 1924-1930

43. Abu-Abied M, Golomb L, Belausov E, Huang S, Geiger B, Kam Z., Staiger CJ and Sadot E. Identification of plant cytoskeleton-interacting proteins by screening for actin stress fiber association in mammalian fibroblasts. Plant J. , 2006, 48, 367-379

44. Thomas S#, Huang S#, Li S, Staiger CJ and Franklin-Tong VE. Actin depolymerization is sufficient to induce programmed cell death in self-incompatible pollen. J. Cell. Biol., 2006, 174, 221-229 (featured as an “In this issue” article, #co-first authors)

45. Huang S, Gao L, Blanchoin L and Staiger CJ. Heterodimeric capping Protein from Arabidopsis is regulated by phosphatidic acid. Mol. Biol. Cell, 2006, 17, 1946-1958

46. Basu D, Le J, El-Essal SED, Huang S, Zhang C, Mallery EL, Koliantz G, Staiger CJ and Szymanski DB. DISTORTED3 is an Arabidopsis WAVE complex subunit that activates ARP2/3 during epidermal morphogenesis. Plant Cell, 2005, 17,502-524

47. Huang S, Robinson RC, Gao LY, Matsumoto T, Brunet A, Blanchoin L and Staiger CJ. Arabidopsis VILLIN1 generates actin filament cables that are resistant to depolymerization. Plant Cell, 2005, 17, 486-501

48. Michelot A#, Guerin C#, Huang S#, Ingouff M, Richard S, Rodiuc N, Staiger CJ and Blanchoin L. The formin homology 1 domain modulates the nucleation and bundling activity of Arabidopsis FORMIN1. Plant Cell, 2005, 17, 2296-2313 (#co-first authors)

49. Xu C#, Huang S# and Yuan M. Dimethyl sulfoxide is feasible for plant tubulin assembly in vitro: a comprehensive analysis. J Integr. Plant Biol. , 2005, 47(4):457-466 (#co-first authors)

50. Huang S, Blanchoin L, Chaudhry F, Franklin-Tong VE and Staiger CJ. A gelsolin-like protein from Papaver rhoeas pollen stimulates calcium-regulated actin severing and depolymerization. J. Biol. Chem. , 2004, 279, 23364-23375 (cover story)

51. Huang S, Blanchoin L, Kovar DR and Staiger CJ. Arabidopsis capping protein (AtCP) is a heterodimer that regulates assembly at the barbed ends of actin filaments. J. Biol. Chem., 2003, 278, 44832-44842

52. Huang S. Ren H and Yuan M. In vitro assembly of plant tubulin in the absence of microtubule-stabilizing reagents. Chinese Sci. Bull. , 2000, 45(24), pp 2258-2263

● 联系方式:

电话:+86-10-62781946

E-mail:sjhuang@tsinghua.edu.cn