方显杨 博士

研究员、青年千人

1998-2002年    武汉大学                                 化学与分子科学学院    学士

2002-2008年    中国科学院生物物理研究所    生物大分子核磁共振    博士

2009-2015年    美国国立卫生研究院国立癌症研究所                          博士后

2015年至今      清华大学生命科学学院                                                研究员、博导

 

● 研究兴趣、领域:

本实验室主要利用小角X射线散射(SAXS)、核磁共振(NMR)、X射线晶体学、计算生物学等方法技术,开展与人类疾病和重大生命过程密切相关的非编码RNA的结构生物学研究,同时发展基于小角X射线散射技术和其它生物物理手段联用的整合结构生物学(Integrated Structural Biology)新方法。

● 代表性论文:

1.      Xianyang Fang, Jason R. Stagno,Yuba Bhandari,Xiaobing Zuo,Yun-Xing Wang. Small-angle X-ray scattering: a bridge between RNA secondary structures and three-dimensional topological structures. Current Opinion in Structural Biology 2015,30:147-160.

2.      Sujay Subbayya Ithychanda, Xianyang Fang, Maradumane L. Mohan, Liang Zhu, Kalyan C. Tirupula, Sathyamangla V. Naga Prasad, Yun-Xing Wang, Sadashiva Karnik, Jun Qin. A mechanism of global shape-dependent recognition and phosphorylation of filamin by protein kinase A. Journal of Biological Chemistry 2015, 290(13):8527-38.

3.      Yi Peng, Joseph E. Curtis, Xianyang Fang, and Sarah A. Woodson. Structural Model of the folded rpoS mRNA leader in complex with Hfq. Proceedings of the Naitonal Academy of Sciences 2014, 111(48): 17134-9.

4.      Alexander Lemak, Bin Wu, Adelinda Yee, Scott Houliston, Hsiau-Wei Lee,  Aleksandras Gutmanas, Xianyang Fang, Maite Garcia, Anthony Semesi,  Yun-Xing Wang, James H. Prestegard, Cheryl H. Arrowsmith. Structural characterization of a flexible two-domain protein in solution based using Small Angle X-ray Scattering and NMR Data. Structure 2014, 22(12):1862-74

5.      Feng-Ming James Chang, Coyne, H. Jerome, Ramirez, Ciro Alberto Cubillas, Pablo Vinuesa Fleischmann, Xianyang Fang, Zhen Ma, Dejian Ma, John D. Helmann, Alejandro García-de los Santos, Yun-Xing Wang, Charles E. Dann III, and David P. Giedroc. Cu(I)-mediated Allosteric Switching in a Copper-sensing Operon Repressor (CsoR). Journal of Biological Chemistry 2014, 289(27):19204-17.

6.      Xianyang Fang# , Jinbu Wang#, Ina P. O’Carroll#, Michelle Mitchell, Xiaobing Zuo, Yi Wang, Ping Yu, Yu Liu, Jason W. Rausch, Marzena A. Dyba, J?rgen Kjems, Charles D. Schwieters, Soenke Seifert, Randall E. Winans, Norman R. Watts, Stephen J. Stahl, Paul T. Wingfield, R. Andrew Byrd, Stuart F. J. Le Grice, Alan Rein*,Yun-Xing Wang*. An unusual topological structure of the HIV-1 rev response element, Cell 2013, 155(3): 594-605.

7.      Moshe Giladi, Itay Friedberg, Xianyang Fang, Reuben Hiller, Yun-Xing Wang,  Daniel Khananshvili. G503 is obligatory for dynamic coupling of regulatory domains in NCX proteins Biochemistry 2012, 51(37):7313-20.

8.      Moshe Giladi, Yeheskel Sasson, Xianyang Fang, Reuben Hiller, Tal Buki, Yun-Xing Wang, Joel Alan Hirsch, Daniel Khananshvili. A common Ca2+-driven interdomain module governs eukaryotic NCX regulation, PLoS One 2012, 7(6): e39985.

9.      George Avvakumov, John Walker, Sheng Xue, Abdellah Allali-Hassani, Abdalin Asinas, Usha Nair, Xianyang Fang, Xiaobing Zuo, Yun-Xing Wang, Keith Wilkinson, Sirano Dhe-Paganon. Two ZnF-UBP domains in isopeptidase T (USP5), Biochemistry 2012, 51(6): 1188-98.

10.  Xianyang Fang, Yingang Feng, and Jinfeng Wang. Favorable contribution of the C-terminal residue K97 to the stability of a hyperthermophilic archaeal [P62A]Ssh10b, Archives of Biochemistry and Biophysics 2009, 481(1):52-8.

11.  Xianyang Fang#, Qiu Cui#, Yufeng Tong, Yingang Feng, Lu Shan, Li Huang, and Jinfeng Wang. A stabilizing a/β-hydrophobic core greatly contributes to hyperthermostability of archaeal [P62A]Ssh10b. Biochemistry 2008, 47(43):11212-21.

12.  Quan Luo#, Xianyang Fang#, Zhiwei Deng, Jinfeng Wang. NMR study of influences of D51N mutation on backbone conformation of hyperthermophilic archaeal [P62A]Ssh10b. Chemical  Journal of Chinese Universities-Chinese 2008,29,546-550.  

13.  Xianyang Fang*, Malgorzata Michnicka, Yikan Zhang, Yun-Xing Wang, and Edward P. Nikonowicz*. Capture and Release of tRNA by the T-Loop Receptor in the Function of the T-Box Riboswitch. Biochemistry. 2017, 56(28):3549-3558.

14.  Smita Kakar#Xianyang Fang#, Lucyna Lubkowska#, Yan Ning Zhou, Gary X. Shaw, Yun-Xing Wang,Ding Jun Jin*, Mikhail Kashlev*, and Xinhua Ji*. Allosteric Activation of Bacterial Swi2/Snf2 (Switch/Sucrose Non-fermentable) Protein RapA by RNA Polymerase: BIOCHEMICAL AND STRUCTURAL STUDIES. Journal of Biological Chemistry 2015, 290 (39):23656-69.

15.  Xianyang Fang, Jason R. Stagno,Yuba Bh.andari,Xiaobing Zuo,Yun-Xing Wang. Small-angle X-ray scattering: a bridge between RNA secondary structures and three-dimensional topological structures. Current Opinion in Structural Biology 2015,30:147-160.

16.  Yi Peng, Joseph E. Curtis, Xianyang Fang, and Sarah A. Woodson. Structural Model of the folded rpoS mRNA leader in complex with Hfq. Proceedings of the Naitonal Academy of Sciences 2014, 111(48): 17134-9.

17.  Xianyang Fang, Jinbu Wang#, Ina P. O’Carroll#, Michelle Mitchell, Xiaobing Zuo, Yi Wang, Ping Yu, Yu Liu, Jason W. Rausch, Marzena A. Dyba, J?rgen Kjems, Charles D. Schwieters, Soenke Seifert, Randall E. Winans, Norman R. Watts, Stephen J. Stahl, Paul T. Wingfield, R. Andrew Byrd, Stuart F. J. Le Grice, Alan Rein*,Yun-Xing Wang*. An unusual topological structure of the HIV-1 rev response element, Cell 2013, 155(3): 594-605.( #equal contribution)

18.  Xianyang Fang, Yingang Feng, and Jinfeng Wang. Favorable contribution of the C-terminal residue K97 to the stability of a hyperthermophilic archaeal [P62A]Ssh10b, Archives of Biochemistry and Biophysics 2009, 481(1):52-8.

19.  Xianyang Fang#, Qiu Cui#, Yufeng Tong, Yingang Feng, Lu Shan, Li Huang, and Jinfeng Wang. A stabilizing a/β-hydrophobic core greatly contributes to hyperthermostability of archaeal [P62A]Ssh10b. Biochemistry 2008, 47(43):11212-21.

 

# co-first author; *corresponding author

 

● 联系方式:

Email: fangxy@mail.tsinghua.edu.cn

Tel: +86-10-62771071