陈春来 博士

研究员、博士生导师

1999-2003    学士      北京大学化学与分子工程学院

2003-2008    博士      北京大学化学与分子工程学院

2008-2015    博士后/研究员   University of Pennsylvania

2015-至今    研究员  清华大学生命科学学院,清华-北大生命科学联合中心(CLS)

 

● 研究兴趣、领域:

1.发展单分子荧光方法和技术;

2.CRISPR系列蛋白靶向和剪切机制的研究;

3.核糖体在蛋白质合成过程中翻译和调控机制的研究。

 

● 代表性论文:

  1. Sijia Peng, Wenjuan Wang, and Chunlai Chen(*), (2018) Surface Transient Binding-Based Fluorescence Correlation Spectroscopy (STB-FCS), a Simple and Easy-to-Implement Method to Extend the Upper Limit of the Time Window to Seconds, J. Phys. Chem. B, Article ASAP, DOI: 10.1021/acs.jpcb.8b03476

  2. Mengyi Yang(#), Sijia Peng(#), Ruirui Sun, Jingdi Lin, Nan Wang, and Chunlai Chen(*), (2018) The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET, Cell Reports, 22(2): 372-382

  3. Sijia Peng, Wenjuan Wang (*) and Chunlai Chen(*), (2018) Breaking the concentration barrier for single molecule fluorescence measurements, Chemistry - A European Journal, 24: 1002-1009

  4. Sijia Peng, Ruirui Sun, Wenjuan Wang and Chunlai Chen(*), (2017) Single-Molecule Photoactivation FRET: A General and Easy-to-Implement Approach to Break the Concentration Barrier, Angew. Chem. Int. Ed, 56(24):6882-6885

  5. Sijia Peng, Mengyi Yang, Rui Ning Sun, Yang Liu, Wenjuan Wang, Qiaoran Xi, Haipeng Gong, and Chunlai Chen(*), (2017) Mechanism of actions of Oncocin, a proline-rich antimicrobial peptide, in early elongation revealed by single-molecule FRET, Protein & Cell, doi: 10.1007/s13238-017-0495-2

  6. Sijia Peng, Ruirui Sun, Wenjuan Wang, and Chunlai Chen(*), (2017) Single-molecule FRET studies on interactions between elongation factor 4 (LepA) and ribosomes, Chinese Chemical Letters, doi: 10.1016/j.cclet.2017.12.006

  7. Ryan M. Jamiolkowski, Chunlai Chen(*), Barry S. Cooperman(*), and Yale E. Goldman(*), (2017) tRNA Fluctuations Observed on Stalled Ribosomes Are Suppressed during Ongoing Protein Synthesis, Biophysical Journal, 113(11):2326-2335

  8. Shan Sun, Lin Li, Fan Yang, Xiaojing Wang, Fenghui Fan, Mengyi Yang, Chunlai Chen, Xueming Li, Hong-Wei Wang, Sen-Fang Sui (*), (2017) Cryo-EM structures of the ATP-bound Vps4E233Q hexamer and its complex with Vta1 at near-atomic resolution, Nature Communications, Jul 17;8:16064

  9. Chunlai Chen, Xiaonan Cui, John F. Beausang, Haibo Zhang, Ian Farrell, Barry S. Cooperman (*), and Yale E. Goldman (*), (2016) Elongation factor G initiates translocation through a power stroke, PNAS, 113(27):7515-20

  10. Wenjuan Wang(*) and Chunlai Chen(*), (2016) Tracking translation of single mRNA molecule in live cells, Sci. Bull. 61(14):1071-1073

  11. Chunlai Chen, Haibo Zhang, Steven L. Broitman, Michael Reiche, Ian Farrell, Barry S. Cooperman(*), and Yale E. Goldman(*), (2013) Dynamics of translation by single ribosomes through mRNA secondary structures, Nature Structural & Molecular Biology, 20, 582-588.

 

● 联系方式:

E-mail: chunlai@biomed.tsinghua.edu.cn