Chunlai CHEN,Ph.D.

Principal Investigator


1999-2003    B.Sc.     Peking University

2003-2008    Ph.D.     Peking University

2008-2015    Postdoc/Research Specialist     University of Pennsylvania

2015-now     Principal Investigator        School of Life Sciences; Tsinghua-Peking Joint Center for Life Sciences; Beijing Advanced Innovation Center for Structural Biology, Tsinghua University

 

Research interest


1) Develop cutting-edge single molecule fluorescence methods and techniques.

2) Molecular mechanisms of CRISPR proteins in target searching and cleavage.

3) Molecular mechanisms of the ribosome and associated proteins during protein synthesis.

 

Selected publications


1.Sijia Peng, Wenjuan Wang, and Chunlai Chen(*), (2018) Surface Transient Binding-Based Fluorescence Correlation Spectroscopy (STB-FCS), a Simple and Easy-to-Implement Method to Extend the Upper Limit of the Time Window to Seconds, J. Phys. Chem. B, Article ASAP, DOI: 10.1021/acs.jpcb.8b03476

2.Mengyi Yang(#), Sijia Peng(#), Ruirui Sun, Jingdi Lin, Nan Wang, and Chunlai Chen(*), (2018) The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET, Cell Reports, 22(2): 372-382

3.Sijia Peng, Wenjuan Wang (*) and Chunlai Chen(*), (2018) Breaking the concentration barrier for single molecule fluorescence measurements, Chemistry - A European Journal, 24: 1002-1009

4.Sijia Peng, Ruirui Sun, Wenjuan Wang and Chunlai Chen(*), (2017) Single-Molecule Photoactivation FRET: A General and Easy-to-Implement Approach to Break the Concentration Barrier, Angew. Chem. Int. Ed, 56(24):6882-6885

5.Sijia Peng, Mengyi Yang, Rui Ning Sun, Yang Liu, Wenjuan Wang, Qiaoran Xi, Haipeng Gong, and Chunlai Chen(*), (2017) Mechanism of actions of Oncocin, a proline-rich antimicrobial peptide, in early elongation revealed by single-molecule FRET, Protein & Cell, doi: 10.1007/s13238-017-0495-2

6.Sijia Peng, Ruirui Sun, Wenjuan Wang, and Chunlai Chen(*), (2017) Single-molecule FRET studies on interactions between elongation factor 4 (LepA) and ribosomes, Chinese Chemical Letters, doi: 10.1016/j.cclet.2017.12.006

7.Ryan M. Jamiolkowski, Chunlai Chen(*), Barry S. Cooperman(*), and Yale E. Goldman(*), (2017) tRNA Fluctuations Observed on Stalled Ribosomes Are Suppressed during Ongoing Protein Synthesis, Biophysical Journal, 113(11):2326-2335

8.Shan Sun, Lin Li, Fan Yang, Xiaojing Wang, Fenghui Fan, Mengyi Yang, Chunlai Chen, Xueming Li, Hong-Wei Wang, Sen-Fang Sui (*), (2017) Cryo-EM structures of the ATP-bound Vps4E233Q hexamer and its complex with Vta1 at near-atomic resolution, Nature Communications, Jul 17;8:16064

9.Chunlai Chen, Xiaonan Cui, John F. Beausang, Haibo Zhang, Ian Farrell, Barry S. Cooperman (*), and Yale E. Goldman (*), (2016) Elongation factor G initiates translocation through a power stroke, PNAS, 113(27):7515-20

10.Wenjuan Wang(*) and Chunlai Chen(*), (2016) Tracking translation of single mRNA molecule in live cells, Sci. Bull. 61(14):1071-1073

11.Chunlai Chen, Haibo Zhang, Steven L. Broitman, Michael Reiche, Ian Farrell, Barry S. Cooperman(*), and Yale E. Goldman(*), (2013) Dynamics of translation by single ribosomes through mRNA secondary structures, Nature Structural & Molecular Biology, 20, 582-588.

 

1.Sijia Peng, Wenjuan Wang, and Chunlai Chen(*), (2018) Surface Transient Binding-Based Fluorescence Correlation Spectroscopy (STB-FCS), a Simple and Easy-to-Implement Method to Extend the Upper Limit of the Time Window to Seconds, J. Phys. Chem. B, Article ASAP, DOI: 10.1021/acs.jpcb.8b03476

2.Mengyi Yang(#), Sijia Peng(#), Ruirui Sun, Jingdi Lin, Nan Wang, and Chunlai Chen(*), (2018) The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET, Cell Reports, 22(2): 372-382

3.Sijia Peng, Wenjuan Wang (*) and Chunlai Chen(*), (2018) Breaking the concentration barrier for single molecule fluorescence measurements, Chemistry - A European Journal, 24: 1002-1009

4.Sijia Peng, Ruirui Sun, Wenjuan Wang and Chunlai Chen(*), (2017) Single-Molecule Photoactivation FRET: A General and Easy-to-Implement Approach to Break the Concentration Barrier, Angew. Chem. Int. Ed, 56(24):6882-6885

5.Sijia Peng, Mengyi Yang, Rui Ning Sun, Yang Liu, Wenjuan Wang, Qiaoran Xi, Haipeng Gong, and Chunlai Chen(*), (2017) Mechanism of actions of Oncocin, a proline-rich antimicrobial peptide, in early elongation revealed by single-molecule FRET, Protein & Cell, doi: 10.1007/s13238-017-0495-2

6.Sijia Peng, Ruirui Sun, Wenjuan Wang, and Chunlai Chen(*), (2017) Single-molecule FRET studies on interactions between elongation factor 4 (LepA) and ribosomes, Chinese Chemical Letters, doi: 10.1016/j.cclet.2017.12.006

7.Ryan M. Jamiolkowski, Chunlai Chen(*), Barry S. Cooperman(*), and Yale E. Goldman(*), (2017) tRNA Fluctuations Observed on Stalled Ribosomes Are Suppressed during Ongoing Protein Synthesis, Biophysical Journal, 113(11):2326-2335

8.Shan Sun, Lin Li, Fan Yang, Xiaojing Wang, Fenghui Fan, Mengyi Yang, Chunlai Chen, Xueming Li, Hong-Wei Wang, Sen-Fang Sui (*), (2017) Cryo-EM structures of the ATP-bound Vps4E233Q hexamer and its complex with Vta1 at near-atomic resolution, Nature Communications, Jul 17;8:16064

9.Chunlai Chen, Xiaonan Cui, John F. Beausang, Haibo Zhang, Ian Farrell, Barry S. Cooperman (*), and Yale E. Goldman (*), (2016) Elongation factor G initiates translocation through a power stroke, PNAS, 113(27):7515-20

10.Wenjuan Wang(*) and Chunlai Chen(*), (2016) Tracking translation of single mRNA molecule in live cells, Sci. Bull. 61(14):1071-1073

11.Chunlai Chen, Haibo Zhang, Steven L. Broitman, Michael Reiche, Ian Farrell, Barry S. Cooperman(*), and Yale E. Goldman(*), (2013) Dynamics of translation by single ribosomes through mRNA secondary structures, Nature Structural & Molecular Biology, 20, 582-588.

 

Contact information

E-mail: chunlai@mail.tsinghua.edu.cn