Shanjin HUANG, Ph.D.

Principal Investigator


Education
1996~2001 Ph.D.,   College of Biological Sciences, China Agricultural University, Beijing, P.R. China
     Major: Plant Sciences
1992~1996  B.S.,   Zhejiang Agricultural University, Zhejiang Province, P.R. China
     Major: Agronomy

Employment
2014~present, Principal Investigator, School of Life Sciences, Tsinghua University.

2006-2016, Principal Investigator, Institute of Botany, Chinese Academy of Sciences

2001-2006, Post-doc fellow, Department of Biological Sciences, Purdue University

 

Research interest


We are interested in dissecting the cellular mechanisms underlying plant growth and development as well as the interaction of plant with its surrounding environment. Specifically, we are focusing on two following topics: 1)Structure, function and regulation of the plant cytoskeleton; 2)Assembly, function and regulation of non-membranous organelles

Selected publications


1. Zhang Y, Li Z, Chen N, Huang Y and Huang S*. Phase Separation of Arabidopsis EMB1579 Controls Transcription, mRNA Splicing and Development. PLOS Biol., 2020,21;18(7):e3000782

2. Zhao W, Qu X, Zhuang Y, Wang L, Bosch M, Franklin-Tong VE, Xue Y, Huang S*. Villin Controls the Formation and Enlargement of Punctate Actin Foci in Pollen Tubes. J Cell Sci., 2020, Mar 18; 133 (6). pii: jcs237404

3. Zhang R, Qu X, Zhang M, Jiang Y, Dai A, Zhao W, Cao D, Lan Y, Yu R, Wang H and Huang S*. The Balance between Actin Bundling Factors Controls Actin Architecture in Pollen Tubes. iScience, 2019, 28;16:162-176

4. Jiang Y, Chang M, Lan Y and Huang S*. Mechanism of CAP1-mediated Apical Actin Polymerization in Pollen Tubes. Proc Natl Acad Sci U S A., 2019;116(24):12084-12093

5. Lan Y, Liu X, Fu Y* and Huang S*. Arabidopsis Class I Formins Control Membrane-Originated Actin Polymerization at Pollen Tube Tips. PLOS Genet. 2018, 14(11): e1007789

6. Diao M, Ren S, Wang Q, Qian L, Shen J, Liu Y and Huang S*. Arabidopsis Formin 2 Regulates Cell-to-Cell Trafficking by Capping and Stabilizing Actin Filaments at Plasmodesmata. eLife 2018;7:e36316

7. Jiang Y, Wang J, Xie Y, Chen N and Huang S* . Arabidopsis ADF10 Shapes the Organization of Apical Actin Filaments via Promoting their Turnover and Ordering in Pollen Tubes. J Cell Sci. 2017, 130, 3988-4001

8. Qu X, Zhang R, Zhang M, Diao M, Xue Y and Huang S* . Organizational Innovation of Apical Actin Filaments Drives Rapid Pollen Tube Growth and Turning. Mol Plant. 2017, 10, 930–947

9. Chang M, Li Z and Huang S*. Monomeric G-actin is uniformly distributed in pollen tubes and is rapidly redistributed via cytoplasmic streaming during pollen tube growth. Plant J., 2017, 92(3): 509-519

10. Jiang Y, Zhang M and Huang S*. Analysis of Actin-based Intracellular Trafficking in Pollen Tubes. Methods Mol Biol., 2017, 1662:125-136

11. Zhang R, Chang M, Zhang M, Wu Y, Qu X and Huang S*. The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins. J Biol Chem., 2016, 9;291(34):17881-96.

12. Zhang M, Zhang R, Qu X and Huang S* . Arabidopsis FIM5 decorates apical actin filaments and regulates their organization in the pollen tube. J Exp Bot., 2016, 67(11):3407-17

13. Liu X, Qu X, Jiang Y, Chang M, Zhang R, Wu Y, Fu Y and Huang S*. Profilin regulates apical actin polymerization to control polarized pollen tube growth. Mol Plant, 2015, 8(12):1694-709 (cover story)

14. Chang M and Huang S* . Arabidopsis ACT11 modifies actin turnover to promote pollen germination and maintain the normal rate of tube growth. Plant J. , 2015, 83 (3):515-527

15. Wu S, Xie Y, Zhang J, Ren Y, Zhang X, Wang J, Guo X, Wu F, Sheng P, Wang J, Wu C, Wang H, Huang S* and Wan J*. VLN2 regulates plant architecture by affecting microfilament dynamics and polar auxin transport in rice. Plant Cell, 2015, 27(10):2829-2845

16. Qu X, Jiang Y, Chang M, Liu X, Zhang R and Huang S*. Organization and regulation of the actin cytoskeleton in the pollen tube. Front. Plant Sci. , 2015, 8;5:786

17. Wang Q and Huang S* . Visualization of microtubules organization and dynamics in living Arabidopsis embryonic cells. Mol. Plant, 2014;7(8):1397-401

18. Zheng Y, Xie Y, Jiang Y, Qu X and Huang S*. Arabidopsis actin depolymerizing factor7 severs actin filaments and regulates actin cable turnover to promote normal pollen tube growth. Plant Cell, 2013, 25:3405-3423

19. Qu X, Zhang H, Xie Y, Wang J, Chen N and Huang S*. Arabidopsis villins promote actin turnover at pollen tube tips and facilitate the construction of actin collars. Plant Cell, 2013, 25:1803-17

20. Shi M, Xie Y, Zheng Y, Wang J, Su Y, Yang Q and Huang S*. Oryza sativa actin-interacting protein 1 is required for rice growth via promoting actin turnover. Plant J. , 2013, 73, 747-760

21. Bao C, Wang J, Zhang R, Zhang B, Zhang H, Zhou Y and Huang S*. Arabidopsis VILLIN2 and VILLIN3 act redundantly in sclerenchyma development via bundling of actin filaments. Plant J. , 2012,71: 962-975

22. Yang W, Ren S, Zhang X, Gao M, Ye S, Qi Y, Zheng Y, Wang J, Zeng L, Qun Li, Huang S*, and He Z*. BENT UPPERMOST INTERNODE1 encodes the class II formin FH5 crucial for actin organization and rice development. Plant Cell, 2011, 23:661-80=

23. Wu Y, Yan J, Zhang R, Qu X, Ren S, Chen N and Huang S* . Arabidopsis FIMBRIN5, an actin bundling factor, is required for pollen germination and pollen tube growth. Plant Cell, 2010, 22: 3745-3763

24. Zhang H, Qu X, Bao C, Khurana P, Wang Q, Xie Y, Zheng Y, Chen N, Blanchoin L, Staiger C.J and Huang S* . Arabidopsis VILLIN5, an actin filament bundling and severing protein, is required for normal pollen tube growth. Plant Cell, 2010, 22: 2749-2767

25. Ye J, Zheng Y, Yan A, Chen N, Wang Z, Huang S* and Yang Z*. Arabidopsis formin3 directs the formation of actin cables and polarized growth in pollen tubes. Plant Cell, 2009, 21:3868–3884

26. Thomas S#, Huang S#, Li S, Staiger CJ and Franklin-Tong VE. Actin depolymerization is sufficient to induce programmed cell death in self-incompatible pollen. J. Cell. Biol., 2006, 174, 221-229 (featured as an “In this issue” article, #co-first authors)

27. Huang S, Gao L, Blanchoin L and Staiger CJ. Heterodimeric capping Protein from Arabidopsis is regulated by phosphatidic acid. Mol. Biol. Cell, 2006, 17, 1946-1958

28. Huang S, Robinson RC, Gao LY, Matsumoto T, Brunet A, Blanchoin L and Staiger CJ. Arabidopsis VILLIN1 generates actin filament cables that are resistant to depolymerization. Plant Cell, 2005, 17, 486-501

29. Michelot A#, Guerin C#, Huang S#, Ingouff M, Richard S, Rodiuc N, Staiger CJ and Blanchoin L. The formin homology 1 domain modulates the nucleation and bundling activity of Arabidopsis FORMIN1. Plant Cell, 2005, 17, 2296-2313 (#co-first authors)

30. Huang S, Blanchoin L, Chaudhry F, Franklin-Tong VE and Staiger CJ. A gelsolin-like protein from Papaver rhoeas pollen stimulates calcium-regulated actin severing and depolymerization. J. Biol. Chem. , 2004, 279, 23364-23375 (cover story)

31. Huang S, Blanchoin L, Kovar DR and Staiger CJ. Arabidopsis capping protein (AtCP) is a heterodimer that regulates assembly at the barbed ends of actin filaments. J. Biol. Chem., 2003, 278, 44832-44842

Contact information


Tel: +86-10-62781946
E-mail: sjhuang@tsinghua.edu.cn