Xianyang FANG, Ph.D.

Principal Investigator


1998- 2002  Wuhan University, Hubei, China  B.S.

2002-2008   Chinese Academy of Sciences, Beijing, China   Ph.D.

2009-2015   National Cancer Institute, National Institute of Health, USA   Postdoc.

2015-Present   School of Life Sciences, Tsinghua University, Beijing, China   PI

 

Research interest


Our research focuses on the structural biology of non-coding RNAs involved in important biological processes and human diseases and functional amyloids implicated in biofilm formation by key techniques including Small angle X-ray scattering (SAXS), NMR, X-ray crystallography and computation, in conjunction with other biophysical, biochemical and molecular biology methods; we are also interested in the development of novel integrative structural methods by combined use of SAXS and other biophysical techniques.

 

Selected publications


1.   Fang X*, Michnicka M, Zhang Y, Wang YX, Nikonowicz EP*. (2017) Capture and Release of tRNA by the T-Loop Receptor in the Function of the T-Box Riboswitch. Biochemistry, 56(28):3549-3558.

2.   Kakar S#, Fang X#, Lubkowska L#, Zhou YN, Shaw GX, Wang YX, Jin DJ*, Kashlev M*, Ji X*. (2015)Allosteric Activation of Bacterial Swi2/Snf2 (Switch/Sucrose Non-fermentable) Protein RapA by RNA Polymerase: BIOCHEMICAL AND STRUCTURAL STUDIES. J Biol Chem, 290 (39):23656-69.

3.   Fang X, Stagno JR, Bhandari YR, Zuo X, Wang YX*. (2015) Small-angle X-ray scattering: a bridge between RNA secondary structures and three-dimensional topological structures. Curr Opin Struct Biol.,30:147-160.

4.   Ithychanda SS#, Fang X, Mohan ML, Zhu L, Tirupula KC, Naga Prasad SV, Wang YX, Karnik SS, Qin J*. (2015) A mechanism of global shape-dependent recognition and phosphorylation of filamin by protein kinase A. J Biol Chem, 290(13):8527-38.

5.   Peng Y#, Curtis JE, Fang X, Woodson SA*. (2014) Structural model of an mRNA in complex with the bacterial chaperone Hfq. Proc Natl Acad Sci U S A. Dec 2;111(48):17134-9.

6.   Fang X#, Wang J#, O'Carroll IP#, Mitchell M, Zuo X, Wang Y, Yu P, Liu Y, Rausch JW, Dyba MA, Kjems J, Schwieters CD, Seifert S, Winans RE, Watts NR, Stahl SJ, Wingfield PT, Byrd RA, Le Grice SF, Rein A#, Wang YX#. (2013)An unusual topological structure of the HIV-1 rev response element, Cell, 155(3): 594-605.( #equal contribution)

7.   Fang X, Feng Y, Wang J*. (2009) Favorable contribution of the C-terminal residue K97 to the stability of a hyperthermophilic archaeal [P62A]Ssh10b, Arch Biochem Biophys., 481(1):52-8.

8.   Fang X#, Cui Q#, Tong Y, Feng Y, Shan L, Huang L, Wang J*. (2008) A stabilizing a/β-hydrophobic core greatly contributes to hyperthermostability of archaeal [P62A]Ssh10b. Biochemistry, 47(43):11212-21.


 

Contact information


Email: fangxy@mail.tsinghua.edu.cn;

fxy@mail.biomed.tsinghua.edu.cn