Yan Chuangye Ph.D.

Principal Investigator


2005-2009  Department of Chemistry, Tsinghua University, China / B.S.

2009-2015  School of Life Sciences, Tsinghua University, China / Ph .D.

2015-2019 School of Life Sciences, Tsinghua University, China / Postdoctoral Fellow

2019-present School of Life Sciences, Tsinghua University, China / Principal Investigator

 

Research interest


1. Methods development in X-ray crystallography and cryo-electron microscopy.

2. Investigation of the structure and function of important membrane proteins.

3. Study on the structure and function of spliceosome in eukaryotic cells and  the splicing mechanism of precursor messenger RNA.

 

Selected publications


[1] Yan, C.*, Wan, R.* & Shi, Y.. Molecular Mechanisms of pre-mRNA Splicing through Structural Biology of the Spliceosome. Cold Spring Harb Perspect Biol 11(2019).

[2] Zhang, X., Zhan, X., Yan, C.*#, Zhang, W., Lei, J. & Shi, Y. Structures of the human spliceosomes before and after release of the ligated exon. Cell Res 29, 274-285 (2019).

[3] Zhan, X., Yan, C.*#, Zhang, X., Lei, J. & Shi, Y. Structures of the human pre-catalytic spliceosome and its precursor spliceosome. Cell Res 28, 1129-1140 (2018).

[4] Bai, R.*, Wan, R.*, Yan, C.*, Lei, J. & Shi, Y. (2018). Structures of the fully assembled Saccharomyces cerevisiae spliceosome before activation. Science, doi:10.1126/science.aau0325.

[5] Zhang, X.*, Yan, C.*#, Zhan, X.*, Li, L.*, Lei, J., and Shi, Y. (2018). Structure of the human activated spliceosome in three conformational states. Cell research, doi:10.1038/cr.2018.14.

[6] Zhan, X.*, Yan, C.*#, Zhang, X.*, Lei, J. & Shi, Y. (2018). Structure of a human catalytic step I spliceosome. Science, doi:10.1126/science.aar6401.

[7] Zhang, X.*, Yan, C.*#, Hang, J.*, Finci, L.I., Lei, J., and Shi, Y. An Atomic Structure of the Human Spliceosome. Cell 169, 918-929 e914, doi:10.1016/j.cell.2017.04.033 (2017).

[8] Yan, C.*, Wan, R.*, Bai, R.*, Huang, G. & Shi, Y. Structure of a yeast step II catalytically activated spliceosome. Science 355, 149-155, doi:10.1126/science.aak9979 (2017).

[9] Wan, R.*, Yan, C.*, Bai, R.*, Lei, J. & Shi, Y. (2017). Structure of an Intron Lariat Spliceosome from Saccharomyces cerevisiae. Cell 171, 120-132 e112.

[10] Bai, R.*, Yan, C.*, Wan, R.*, Lei, J. & Shi, Y. (2017). Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae. Cell 171, 1589-1598.

[11] Yan, C.*, Wan, R.*, Bai, R.*, Huang, G. & Shi, Y. (2016). Structure of a yeast activated spliceosome at 3.5 A resolution. Science 353, 904-911,

[12] Wan, R.*, Yan, C.*, Bai, R.*, Wang, L., Huang, M., Wong, C.C., and Shi, Y. (2016). The 3.8 A structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysis. Science 351, 466-475, doi:10.1126/science.aad6466 (2016).

[13] Wan, R.*, Yan, C.*, Bai, R.*, Huang, G.* & Shi, Y. (2016) Structure of a yeast catalytic step I spliceosome at 3.4 A resolution. Science 353, 895-904, doi:10.1126/science.aag2235 (2016).

[14] Yan, C.*, Hang, J.*, Wan, R.*, Huang, M., Wong, C.C., and Shi, Y. (2015). Structure of a yeast spliceosome at 3.6-angstrom resolution. Science 349, 1182-1191, doi:10.1126/science.aac7629 (2015).

[15] Hang, J.*, Wan, R.*, Yan, C.* & Shi, Y. (2015). Structural basis of pre-mRNA splicing. Science 349, 1191-1198, doi:10.1126/science.aac8159.

[16] Yan, Z.*, Bai, X.*, Yan, C.*, Wu, J., Li, Z., Xie, T., Peng, W., Yin, C., Li, X., Scheres, S.H.W., et al. (2015). Structure of the rabbit ryanodine receptor RyR1 at near-atomic resolution. Nature 517, 50-55.

[17] Bai, X.C.*, Yan, C.*, Yang, G.*, Lu, P., Ma, D., Sun, L., Zhou, R., Scheres, S.H.W., and Shi, Y. (2015). An atomic structure of human gamma-secretase. Nature 525, 212-217.

[18] Xie, T.*, Yan, C.*, Zhou, R., Zhao, Y., Sun, L., Yang, G., Lu, P., Ma, D., and Shi, Y. (2014). Crystal structure of the gamma-secretase component nicastrin. Proc Natl Acad Sci USA 111, 13349-13354.

[19] Deng D.*, Yan C.*, Pan X.*, Mahfouz M., Wang J., Zhu J.K., Shi Y., Yan N. (2012a) Structural basis for sequence-specific recognition of DNA by TAL effectors. Science 335: 720-723.

*: Co-first author; #: Corresponding authors

 

Chuangye Yan

Contact information


E-mail:yancy2019@mail.tsinghua.edu.cn 

Address:北京市清华大学生命科学学院,闫创业实验室 100084