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生命学院校级平台及高精尖技术中心邀请中科院生物物理所李栋博士于12月28日下午2点在生物新馆143做关于超分辨成像讲座。

李栋博士是国家“青年千人计划”、中科院“百人计划”技术英才类获得者,从事生物光学成像技术开发与应用,特别是适于活体,高速,低漂白成像的超分辨率显微镜,以及多光子荧光组织成像技术,并结合单分子成像与追踪。欢迎大家报名参加!

时间:2017年12月28日   14:00-15:00

地点:生物新馆143

报名邮箱:majialu@biomed.tsinghua.edu.cn    马佳璐      

报名方式:按如下格式发送邮件报名—姓名+学号+手机号+单位院系+课题组

报名截止:2017年12月27日下午17:00

Pushing the Envelope of Super-resolution Live Cell Imaging

Dong Li

Institute of Biophysics, Chinese Academy of Sciences

Super-resolution (SR) fluorescence microscopy provides the capability of resolving ultra-structure beyond the Abbe limit, but many SR methods encounter practical limitations when imaging nano-scale dynamics in living biological samples, especially for long durations over large fields of view. Structured illumination microscopy (SIM) stands out in the context of live imaging, since many fewer raw images and much lower light levels are required. In addition, its higher efficiency in transmitting spatial frequencies beyond the Abbe limit makes it much less photon demanding than other SR methods. However, it typically offers only a two-fold resolution gain, i.e., around 100 nm for green fluorescent protein. Here I will present the latest development of how to extend SIM resolution into sub-100 nm regime, and simultaneously improve the temporal resolution to hundreds of frames per second; as well as the biology uncovered at this high spatiotemporal resolution level.

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