教职员工
兼职教授
讲席教授
杰出人才
其他获奖
陈春来

陈春来,博士

研究员、博士生导师

 

教育和工作经历

1999-2003    学士      北京大学化学与分子工程学院

2003-2008    博士      北京大学化学与分子工程学院

2008-2015    博士后/研究员   University of Pennsylvania

2015-至今    研究员  清华大学生命科学学院,清华-北大生命科学联合中心(CLS),结构生物学高精尖中心

 

主要研究方向

1)发展单分子荧光方法和技术;

2)CRISPR系列蛋白靶向和剪切机制的研究;

3)核糖体在蛋白质合成过程中翻译和调控机制的研究。

 

代表性科研论文

1.      Mengyi Yang(#), Sijia Peng(#), Ruirui Sun, Jingdi Lin, Nan Wang, and Chunlai Chen(*), (2018) The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET, Cell Reports, 22(2): 372-382

2.      Sijia Peng, Ruirui Sun, Wenjuan Wang and Chunlai Chen(*), (2017) Single-Molecule Photoactivation FRET: A General and Easy-to-Implement Approach to Break the Concentration Barrier, Angew. Chem. Int. Ed, 56(24):6882-6885

3.      Sijia Peng, Wenjuan Wang (*) and Chunlai Chen(*), (2017) Breaking the concentration barrier for single molecule fluorescence measurements, Chemistry - A European Journal, Oct 17. doi: 10.1002/chem.201704065 (Concept)

4.      Sijia Peng, Mengyi Yang, Rui Ning Sun, Yang Liu, Wenjuan Wang, Qiaoran Xi, Haipeng Gong, and Chunlai Chen(*), (2017) Mechanism of actions of Oncocin, a proline-rich antimicrobial peptide, in early elongation revealed by single-molecule FRET, Protein & Cell, doi: 10.1007/s13238-017-0495-2

5.      Sijia Peng, Ruirui Sun, Wenjuan Wang, and Chunlai Chen(*), (2017) Single-molecule FRET studies on interactions between elongation factor 4 (LepA) and ribosomes, Chinese Chemical Letters, doi: 10.1016/j.cclet.2017.12.006

6.      Shan Sun, Lin Li, Fan Yang, Xiaojing Wang, Fenghui Fan, Mengyi Yang, Chunlai Chen, Xueming Li, Hong-Wei Wang, Sen-Fang Sui (*), (2017) Cryo-EM structures of the ATP-bound Vps4E233Q hexamer and its complex with Vta1 at near-atomic resolution, Nature Communications, Jul 17;8:16064

7.      Chunlai Chen, Xiaonan Cui, John F. Beausang, Haibo Zhang, Ian Farrell, Barry S. Cooperman (*), and Yale E. Goldman (*), (2016) Elongation factor G initiates translocation through a power stroke, PNAS, 113(27):7515-20

8.      Chunlai Chen, Haibo Zhang, Steven L. Broitman, Michael Reiche, Ian Farrell, Barry S. Cooperman(*), and Yale E. Goldman(*), (2013) Dynamics of translation by single ribosomes through mRNA secondary structures, Nature Structural & Molecular Biology, 20, 582-588.

9.      Chunlai Chen, Benjamin Stevens, Jaskiran Kaur, Zeev Smilansky, Barry S. Cooperman(*), and Yale E. Goldman(*), (2011) Allosteric vs. Spontaneous E-site tRNA Dissociation Early in Protein Synthesis, PNAS, 108, 16980-16985.

10.   Chunlai Chen, Benjamin Stevens, Jaskiran Kaur, Diana Cabral, Hanqing Liu, Yuhong Wang, Haibo Zhang, Gabriel Rosenblum, Zeev Smilansky, Yale E. Goldman(*), and Barry S. Cooperman(*), (2011) Single Molecule Fluorescence Measurements of Ribosomal Translocation Dynamics. Molecular Cell, 42, 367-377.

 

联系方式

E-mail: chunlai@biomed.tsinghua.edu.cn

 

 
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