教职员工
兼职教授
讲席教授
杰出人才
其他获奖
陈春来

陈春来,博士

研究员、博士生导师

教育和工作经历

1999-2003    学士      北京大学化学与分子工程学院

2003-2008    博士      北京大学化学与分子工程学院

2008-2015    博士后/研究员   University of Pennsylvania

2015-至今    研究员  清华大学生命科学学院,清华-北大生命科学联合中心(CLS)

主要研究方向

1)发展单分子荧光方法和技术;

2)核糖体在蛋白质合成过程中翻译和调控机制的研究;

3)CRISPR系列蛋白靶向和剪切机制的研究。

代表性科研论文

1.     Chunlai Chen, Xiaonan Cui, John F. Beausang, Haibo Zhang, Ian Farrell, Barry S. Cooperman (*), and Yale E. Goldman (*), (2016) Elongation factor G initiates translocation through a power stroke, PNAS, 113(27):7515-20

2.     Wei Liu, Chunlai Chen, Darius Kavaliauskas, Charlotte R. Knudsen, Yale E. Goldman (*) and Barry S. Cooperman (*), (2015) EF-Tu dynamics during pre-translocation complex formation: EF-Tu·GDP exits the ribosome via two different pathways, Nucleic Acids Res., 43(19):9519-28

3.     Wenjuan Wang, Tianqi Li, Klara Felsovalyi, Chunlai Chen, Timothy Cardozo, and Michelle Krogsgaard (*) (2014) Quantitative analysis of T cell receptor complex interaction sites using genetically encoded photo-cross-linkers, ACS Chem Biol., 9(9):2165-72

4.     Chunlai Chen, Haibo Zhang, Steven L. Broitman, Michael Reiche, Ian Farrell, Barry S. Cooperman(*), and Yale E. Goldman(*), (2013) Dynamics of translation by single ribosomes through mRNA secondary structures, Nature Structural & Molecular Biology, 20, 582-588.

5.     Gabriel Rosenblum, Chunlai Chen, Jaskiran Kaur, Xiaonan Cui, Haibo Zhang, Haruichi Asahara, Shaorong Chong, Zeev Smilansky, Yale E. Goldman(*), and Barry S. Cooperman(*), (2012) Quantifying Elongation Rhythm during Full-Length Protein Synthesis, Journal of the American Chemical Society, 135(30), 11322-11329.

6.     Chunlai Chen, Michael J. Greenberg, Joseph M. Laakso, E. Michael Ostap, Yale E. Goldman(*) and Henry Shuman(*), (2012) Kinetic schemes for post-synchronized single molecule dynamics, Biophysical Journal, 102, L23-L25

7.     Gabriel Rosenblum, Chunlai Chen, Jaskiran Kaur, Xiaonan Cui, Yale E. Goldman(*), and Barry S. Cooperman(*), (2012) Real-time assay for testing components of protein synthesis, Nucleic Acids Research, 40(12), e88

8.     Benjamin Stevens(#), Chunlai Chen(#), Ian Farrell, Haibo Zhang, Jaskiran Kaur, Steven L. Broitman, Zeev Smilansky, Barry S. Cooperman, and Yale E. Goldman(*), (2012) FRET-based identification of mRNAs undergoing translation, Plos One, 7(5), e38344

9.     Chunlai Chen, Benjamin Stevens, Jaskiran Kaur, Zeev Smilansky, Barry S. Cooperman(*), and Yale E. Goldman(*), (2011) Allosteric vs. Spontaneous E-site tRNA Dissociation Early in Protein Synthesis, PNAS, 108, 16980-16985.

10.   Chunlai Chen, Benjamin Stevens, Jaskiran Kaur, Diana Cabral, Hanqing Liu, Yuhong Wang, Haibo Zhang, Gabriel Rosenblum, Zeev Smilansky, Yale E. Goldman(*), and Barry S. Cooperman(*), (2011) Single Molecule Fluorescence Measurements of Ribosomal Translocation Dynamics. Molecular Cell, 42, 367-377.

 

联系方式

E-mail: chunlai@biomed.tsinghua.edu.cn

 
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