Junmin Pan, Ph.D.
Professor in Cell Biology
School of Life Sciences
Haidian district, Beijing 100084
P. R. China
HeBei Normal University, B.A., 1986
Institute of Hydrobiology, Chinese Academy of Sciences,M.S., 1989
University of Freiburg, Germany, Ph.D., 1996
UT Southwestern Medical Center, Postdoctora, 1997-1999
UT Southwestern Medical Center, Instructor, 1999-2001
UT Southwestern Medical Center, Research Assistant Prof., 2001-2006
Tsinghua University, Professor, 2006 - present
Cilia and eukaryotic flagella (interchangeable terms) are microtubule-based cellular surface protrusions. They play essential roles in cell motility, signaling to regulate various biological processes including sensory transduction, development, tissues and organ homeostasis. Thus, defects in the structure and/or function of cilia/flagella have been implicated in various human disorders including renal diseases, respiratory disorders, developmental abnormality and even diabetes. The structural integrity and proper function of cilia/flagella requires coordinated regulation of the assembly and disassembly of cilia.
We are using Chlamydomonas, a uni-cellular green alga, as a model organism to study the mechanisms underlying the assembly and disassembly of cilia. Previously we have identified an aurora-like protein kinase - CALK, which is required for flagellar disassembly. Recently, we discovered that microtubule depolymerizing kinesin-CrKinesin13 is phosphorylated and is involved in flagellar assembly and disassembly. Currently we focus on the regulation control of CALK and CrKinesin13. Since cilia disassembly occurs prior to cell division, we also aim to understand how cilia disassembly regulates the cell cycle progression. In addition, we are characterizing several mutants defective in the assembly and disassembly of flagella. These defective genes may be involved in the regulation of Intraflagellar transport, a bidirectional transport process between the flagellar tip and the cell body, which is required for flagellar assembly and disassembly. Since cilia and eukaryotic flagella are conserved cellular organelles, our research findings will have great impact on understanding cilia-related human disorders and may provide guidance on possible diagnostics and treatment for these diseases.
1. Zhangfeng Hu, Yinwen Liang, Wei He and Junmin Pan*. 2015. Cilia disassembly with two distinct phases of regulation. Cell Reports 10, 1803-1810.
2. Zhangfeng Hu, Yinwen Liang, Dan Meng, Liang Wang and Junmin Pan*. 2015. Microtubule Depolymerizing Kinesins in the Regulation of Assembly, Disassembly and Length of Cilia and Flagella. International Review of Cell and Molecular Biology. (invited review), in press
3. Yinwen Liang, Yunong Pang, Qiong Wu, Zhangfeng Hu, Xue Han, Yisheng Xu, Haiteng Deng and Junmin Pan*. 2014. FLA8/KIF3B phosphorylation regulates kinesin-II interaction with IFT-B to control IFT entry and turnaround. Dev Cell. 30, 585-597.
4. Junmin Pan and William Snell. 2014. Organelle size: a cilium length signal regulates IFT cargo loading. Curr. Biol. 24, R875-78.
5. Meng D., Cao M., Oda T., and Junmin Pan* 2014. The conserved ciliary protein Bug22 controls planar beating of Chlamydomonas flagella. J. Cell Sci. 127, 281-287.
6. Cao M., D. Meng, L. Wang, S. Bei, W.J. Snell*, and Junmin Pan*. 2013. Activation loop phosphorylation of a protein kinase is a molecular marker of organelle size that dynamically reports flagellar length. PNAS, 110, 12337-42.
7. Liang Y, Junmin Pan*. 2013. Regulation of Flagellar Biogenesis by a Calcium Dependent Protein Kinase in Chlamydomonas reinhardtii. PLoS ONE 8(7): e69902. doi:10.1371/journal.pone.0069902
8. Wang L., T. Piao, M. Cao, Q. Tao, L. Huang, H. Deng, T. Mao and Junmin Pan*. 2013. Flagellar regeneration requires cytoplasmic microtubule depolymerization and kinesin13. J. Cell Sci. 126, 1531-1540, covered image and featured article
9. Junmin Pan*, T Seeger-Nukpezah, EA Golemis*. 2013. The roles of the cilium in normal and abnormal cell cycles: emphsis on renal cystic pathologies. Cell. Mol. Life Sci. 70, 1849-1874.
10. Junmin Pan*, B Naumann-Bausch, L Wang, M Specht, M Scholz, K Trompelt and M Hippler*. 2011. Protein phosphorylation is a key event of flagellar disassembly revealed by analysis of flagellar phosphoproteins during flagellar shortening in Chlamydomonas. Journal of Proteome Research, 10, 3830-3839.(IF 5.1)
11. Luo M, M Cao, Y Kan, G Li, W Snell and Junmin Pan*. 2011. Protein phosphosphorylation states of an auroa-like kinase marks the length of growing flagella. Current Biology£¬21£¬586-591. (IF 10.9)
12. Piao tian, , Minna Luo, Liang Wang, Yan Guo£¬De Li, Peng Li, William J. Snell£¬ Junmin Pan*. 2009. A microtubule depolymerizing kinesin functions during both flagellar disassembly and flagellar assembly in Chlamydomonas. PNAS, 106, 4713-4718 (direct submission).(IF 9.7)