Full time faculty

Adjunct professor

Chair professor

Administration

Chunlai Chen

Chunlai Chen, Ph.D.

Principal Investigator

 

Education:

1999-2003    B.Sc.     Peking University

2003-2008    Ph.D.     Peking University

2008-2015    Postdoc/Research Specialist     University of Pennsylvania

2015-now     Principal Investigator       School of Life Sciences; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University

 

Research Area:

1) Develop cutting-edge single molecule fluorescence methods and techniques.

2) Molecular mechanisms of the ribosome and associated proteins during protein synthesis.

3) Molecular mechanisms of CRISPR proteins in target searching and cleavage.

 

Selected Publications:

1.     Chunlai Chen, Xiaonan Cui, John F. Beausang, Haibo Zhang, Ian Farrell, Barry S. Cooperman (*), and Yale E. Goldman (*), (2016) Elongation factor G initiates translocation through a power stroke, PNAS, 113(27):7515-20

2.     Wei Liu, Chunlai Chen, Darius Kavaliauskas, Charlotte R. Knudsen, Yale E. Goldman (*) and Barry S. Cooperman (*), (2015) EF-Tu dynamics during pre-translocation complex formation: EF-Tu·GDP exits the ribosome via two different pathways, Nucleic Acids Res., 43(19):9519-28

3.     Wenjuan Wang, Tianqi Li, Klara Felsovalyi, Chunlai Chen, Timothy Cardozo, and Michelle Krogsgaard (*) (2014) Quantitative analysis of T cell receptor complex interaction sites using genetically encoded photo-cross-linkers, ACS Chem Biol., 9(9):2165-72

4.     Chunlai Chen, Haibo Zhang, Steven L. Broitman, Michael Reiche, Ian Farrell, Barry S. Cooperman(*), and Yale E. Goldman(*), (2013) Dynamics of translation by single ribosomes through mRNA secondary structures, Nature Structural & Molecular Biology, 20, 582-588.

5.     Gabriel Rosenblum, Chunlai Chen, Jaskiran Kaur, Xiaonan Cui, Haibo Zhang, Haruichi Asahara, Shaorong Chong, Zeev Smilansky, Yale E. Goldman(*), and Barry S. Cooperman(*), (2012) Quantifying Elongation Rhythm during Full-Length Protein Synthesis, Journal of the American Chemical Society, 135(30), 11322-11329.

6.     Chunlai Chen, Michael J. Greenberg, Joseph M. Laakso, E. Michael Ostap, Yale E. Goldman(*) and Henry Shuman(*), (2012) Kinetic schemes for post-synchronized single molecule dynamics, Biophysical Journal, 102, L23-L25

7.     Gabriel Rosenblum, Chunlai Chen, Jaskiran Kaur, Xiaonan Cui, Yale E. Goldman(*), and Barry S. Cooperman(*), (2012) Real-time assay for testing components of protein synthesis, Nucleic Acids Research, 40(12), e88

8.     Benjamin Stevens(#), Chunlai Chen(#), Ian Farrell, Haibo Zhang, Jaskiran Kaur, Steven L. Broitman, Zeev Smilansky, Barry S. Cooperman, and Yale E. Goldman(*), (2012) FRET-based identification of mRNAs undergoing translation, Plos One, 7(5), e38344

9.     Chunlai Chen, Benjamin Stevens, Jaskiran Kaur, Zeev Smilansky, Barry S. Cooperman(*), and Yale E. Goldman(*), (2011) Allosteric vs. Spontaneous E-site tRNA Dissociation Early in Protein Synthesis, PNAS, 108, 16980-16985.

10.   Chunlai Chen, Benjamin Stevens, Jaskiran Kaur, Diana Cabral, Hanqing Liu, Yuhong Wang, Haibo Zhang, Gabriel Rosenblum, Zeev Smilansky, Yale E. Goldman(*), and Barry S. Cooperman(*), (2011) Single Molecule Fluorescence Measurements of Ribosomal Translocation Dynamics. Molecular Cell, 42, 367-377.

 

Contact

E-mail: chunlai@biomed.tsinghua.edu.cn



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