Full time faculty

Adjunct professor

Chair professor

Administration

Hongwei Wang

Hong-Wei Wang Ph.D.

Professor

 

1992-1996    B.S., Department of Biological Sciences and Biotechnology, Tsinghua University

1996-2001    Ph.D., Department of Biological Sciences and Biotechnology, Tsinghua University

2001-2006    Postdoctoral Fellow, Life Sciences Division, Lawrence Berkeley National Laboratory, USA

2006-2008    Research Scientist, Life Sciences Division, Lawrence Berkeley National Laboratory, USA

2009-2011    Tenure-Track Assistant Professor, Department of Molecular Biophysics and Biochemistry, Yale University, USA

2010.12-      Professor, School of Life Sciences, Tsinghua University

 

Research Interest

Our research group is devoted to elucidating the architecture and mechanism of macromolecular complexes and assemblies with cryo-electron microscopy. More specifically, we are interested in:

1. Structure and mechanism of interactions between cytoskeleton and membrane systems;

2. Structure and mechanism of macromolecular complexes in RNA metabolism;

3. Cryo-electron microscopy new method development and application.

 

Representative Publications

1. Song W, Wang J, Han Z, Zhang Y, Zhang H, Wang W, Chang J, Xia B, Fan S, Zhang D, Wang J*, Wang HW*, and Chai J*. (2015) Structural basis for specific recognition of single-stranded RNA by Toll-like receptor 13. Nat. Struct. Mol. Biol. 22, 782-787.

2. Zhou Q, Huang X, Sun S, Li X, Wang HW*, and Sui SF*. (2015) Cryo-EM structure of SNAP-SNARE assembly in 20S particle. Cell Research 25, 551-560.

3. Chang S, Sun D, Liang H, Wang J, Li J, Guo L, Wang X, Guan C, Boruah BM, Yuan L, Feng F, Yang M, Wang L, Wang Y, Wojdyla J, Li L, Wang J, Wang M, Cheng G, Wang HW*, and Liu Y*. (2015) Cryo-EM Structure of Influenza Virus RNA Polymerase Complex at 4.3 Å Resolution. Mol. Cell 57, 925-935.

4. Liu JJ, Bratkowski MA, Liu X, Niu CY, Ke A*, and Wang HW*. (2014) Visualization of distinct substrate-recruitment pathways in the yeast exosome by EM. Nat. Struct. Mol. Biol. 21, 95-102.

5. Li Y, Hsin J, Zhao L, Cheng Y, Shang W, Huang KC, Wang HW, and Ye S*. (2013) FtsZ protofilaments use a hinge-opening mechanism for constrictive force generation. Science 341, 392-295.

6. Taylor DW, Ma E, Shigematsu H, Cianfrocco MA, Noland CL, Nagayama K, Nogales E, Doudna JA*, and Wang HW*. (2013) Substrate-specific structural rearrangements of human Dicer. Nat. Struct. Mol. Biol. 20, 662-670.

7. Chen X, Taylor DW, Fowler CC, Galan JE, Wang HW, and Wolin SL*. (2013) An RNA degradation machine sculpted by Ro autoantigen and noncoding RNA. Cell 153, 166-177.

8. Shen QT, Hsiue PP, Sindelar CV, Welch MD, Campellone KG*, and Wang HW*. (2012) Structural insight into WHAMM-mediated cytoskeletal coordination during membrane remodeling. J. Cell Biol. 199, 111-124.

9. Zhou K, Kanai R, Lee P, Wang HW*, and Modis Y*. (2012) Toll-like receptor 5 forms asymmetric dimers in the absence of flagellin. J. Struct. Biol. 177, 402-409.

10. Liu XQ and Wang HW*. (2011) Single particle electron microscopy reconstruction of the exosome complex using the random conical tilt method. J. Vis. Exp., doi: 10.3791/2574.

11. Wu X, Shen QT, Stokes N, Lu C, Zheng Q, Polak L, Wang HW, and Fuchs E*. (2011) Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3β. Cell 144, 341-352.

12. Wang HW*, Noland C, Siridechadilok B, Taylor DW, Ma E, Felderer K, Doudna JA*, and Nogales E*. (2009) Structural insights into RNA processing by the human RISC-loading complex. Nat. Struct. Mol. Biol. 16, 1148-1153.

13. Wang HW, Long S, Ciferri C, Westermann S, Drubin DG, Barnes G, and Nogales E*. (2008) Architecture and flexibility of the yeast Ndc80 kinetochore complex. J. Mol. Biol. 383, 894-903.

14. Wang HW*, Wang J, Ding F, Callahan K, Bratkowski MA, Bulter JS, Nogales E, and Ke A*. (2007) Architecture of the yeast Rrp44-exosome complex suggests routes of RNA recruitment for 3’-end processing. Proc. Natl. Acad. Sci. USA 104, 16844-16849.

15. Wang HW, Ramey VH, Westermann S, Leschziner A, Welburn JPI, Nakajima Y, Drubin DG, Barnes G, and Nogales E*. (2007) Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanisms. Nat. Struct. Mol. Biol. 14, 721-726.

16. Nogales E* and Wang HW. (2006) Structural intermediates in microtubule assembly and disassembly: how and why? Curr. Opin. Cell Biol. 18, 179-284.

17. Westermann W, Wang HW, Avila-Sakar A, Drubin DG, Nogales E, and Barnes G*. (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440, 565-569.

18. Wang HW and Nogales E*. (2005) The nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly. Nature 435, 911-915.

 

Contact

Tel£º+86-10-62772390£¨office£©

+86-10-62798651£¨lab£©
E-mail: hongweiwang@tsinghua.edu.cn

Web: http://cryoem.life.tsinghua.edu.cn/

 



Tsinghua University,Beijing,China,100084
Tel:+86-10-62788604  Fax:+86-10-62788604  Email:admin-life@biomed.tsinghua.edu.cn