Full time faculty

Adjunct professor

Chair professor


Hongwei Wang

Hong-Wei Wang, Ph.D.


Young 1000-Talent Plan


1992-1996    B.S., Department of Biological Sciences and Biotechnology, Tsinghua University

1996-2001    Ph.D., Department of Biological Sciences and Biotechnology, Tsinghua University

2001-2006    Postdoctoral Fellow, Life Sciences Division, Lawrence Berkeley National Laboratory, USA

2006-2008    Research Scientist, Life Sciences Division, Lawrence Berkeley National Laboratory, USA

2009-2011    Tenure-Track Assistant Professor, Department of Molecular Biophysics and Biochemistry, Yale University, USA

2010.12-      Professor, School of Life Sciences, Tsinghua University

2016.04-      Dean, School of Life Sciences, Tsinghua University

Research Interest

Our research group is devoted to elucidating the architecture and mechanism of macromolecular complexes and assemblies with cryo-electron microscopy. More specifically, we are interested in:

1. Structure and mechanism of interactions between cytoskeleton and membrane systems;

2. Structure and mechanism of macromolecular complexes in RNA metabolism;

3. Cryo-electron microscopy new method development and application.

Representative Publications

  1. Xu J, Zhao L, Xu Y, Zhao W, Sung P*, and Wang HW*. (2017) Cryo-EM structures of human RAD51 recombinase filaments during catalysis of DNA-strand exchange. Nat. Struct. Mol. Biol., 24:40-46
  2. Wang HW*, and Wang JW. (2017) How cryo-electron microscopy and X-ray crystallography complement each other. Protein Sci., 26:32-39 (Review)
  3. Wang HW*, Lei J*, and Shi Y*. (2017) Biological cryo-electron microscopy in China. Protein Sci., 26:16-31 (Review)
  4. Yang H, Wang J, Liu M, Chen X, Huang M, Tan D, Dong MQ, Wong CC, Wang J*, Xu Y*, and Wang HW*. (2016) 4.4 Å Resolution Cryo-EM structure of human mTOR Complex 1. Protein Cell, 7:878-887
  5. Agrawal RK*, Wang HW*, and Belfort M*. (2016) Forks in the tracks: Group II introns, spliceosomes, telomeres and beyond. RNA Biol., 13:1218-1222
  6. Liu JJ, Niu CY, Wu Y, Tan D, Wang Y, Ye MD, Liu Y, Zhao W, Zhou K, Liu QS, Dai J, Yang X, Dong MQ, Huang N, and Wang HW*. (2016) CryoEM structure of yeast cytoplasmic exosome complex. Cell Research, 26:822-837
  7. Qu G, Kaushal PS, Wang J, Shigematsu H, Piazza CL, Agrawal RK*, Belfort M*, and Wang HW*. (2016) Structure of a group II intron in complex with its reverse transcriptase. Nat. Struct. Mol. Biol., 23:549-557
  8. Qin S, Yin H, Yang C, Dou Y, Liu Z, Zhang P, Yu H, Huang Y, Feng J, Hao J, Hao J, Deng L, Yan X, Dong X, Zhao Z, Jiang T, Wang HW, Luo SJ, and Xie C*. (2016) A magnetic protein biocompass. Nat Mater., 15:217-226
  9. Jia N, Liu N, Cheng W, Jiang YL, Sun H, Chen LL, Peng J, Zhang Y, Ding YH, Zhang ZH, Wang X, Cai G, Wang J, Dong MQ, Zhang Z, Wu H, Wang HW*, Chen Y*, and Zhou CZ*. (2016) Structural basis for receptor recognition and pore formation of a zebrafish aerolysin-like protein. EMBO Reports, 17:235-248
  10. Wang J, Wang W, Song W, Han Z, Zhang H, Chai J*, Wang HW*, and Wang J*. (2015) An improved method for phasing crystal structures with low non-crystallographic symmetry using cryo-electron microscopy data. Protein Cell, 6:919-923 (Letter)
  11. Wang HW*. (2015) Opening new doors for understanding eukaryotic RNA splicing. Sci China Life Sci., 58:1171-1172 (Editorial Material)
  12. Hu Z, Zhou Q, Zhang C, Fan S, Cheng W, Zhao Y, Shao F, Wang HW, Sui SF*, and Chai J*. (2015) Structural and biochemical basis for induced self-propagation of NLRC4. Science, 350:399-404
  13. Tomko RJ Jr*, Taylor DW, Chen ZA, Wang HW, Rappsilber J, and Hochstrasser M*. (2015) A single α helix drives extensive remodeling of the proteasome lid and completion of regulatory particle assembly. Cell, 163:432-444
  14. Song W, Wang J, Han Z, Zhang Y, Zhang H, Wang W, Chang J, Xia B, Fan S, Zhang D, Wang J*, Wang HW*, and Chai J*. (2015) Structural basis for specific recognition of single-stranded RNA by Toll-like receptor 13. Nat. Struct. Mol. Biol., 22, 782-787.
  15. Wang HW*. (2015) Cryo-electron microscopy for structural biology: current status and future perspectives. Sci. China Life Sci., 58:750-756 (Review)
  16. Zhou Q, Huang X, Sun S, Li X, Wang HW*, and Sui SF*. (2015) Cryo-EM structure of SNAP-SNARE assembly in 20S particle. Cell Research, 25:551-560.
  17. Chang S, Sun D, Liang H, Wang J, Li J, Guo L, Wang X, Guan C, Boruah BM, Yuan L, Feng F, Yang M, Wang L, Wang Y, Wojdyla J, Li L, Wang J, Wang M, Cheng G, Wang HW*, and Liu Y*. (2015) Cryo-EM Structure of Influenza Virus RNA Polymerase Complex at 4.3 Å Resolution. Mol. Cell, 57:925-935.
  18. Liu JJ, Bratkowski MA, Liu X, Niu CY, Ke A*, and Wang HW*. (2014) Visualization of distinct substrate-recruitment pathways in the yeast exosome by EM. Nat. Struct. Mol. Biol., 21:95-102.
  19. Li Y, Hsin J, Zhao L, Cheng Y, Shang W, Huang KC, Wang HW, and Ye S*. (2013) FtsZ protofilaments use a hinge-opening mechanism for constrictive force generation. Science, 341:392-295.
  20. Taylor DW, Ma E, Shigematsu H, Cianfrocco MA, Noland CL, Nagayama K, Nogales E, Doudna JA*, and Wang HW*. (2013) Substrate-specific structural rearrangements of human Dicer. Nat. Struct. Mol. Biol., 20:662-670.
  21. Chen X, Taylor DW, Fowler CC, Galan JE, Wang HW, and Wolin SL*. (2013) An RNA degradation machine sculpted by Ro autoantigen and noncoding RNA. Cell, 153:166-177.
  22. Shen QT, Hsiue PP, Sindelar CV, Welch MD, Campellone KG*, and Wang HW*. (2012) Structural insight into WHAMM-mediated cytoskeletal coordination during membrane remodeling. J. Cell Biol., 199:111-124.
  23. Zhou K, Kanai R, Lee P, Wang HW*, and Modis Y*. (2012) Toll-like receptor 5 forms asymmetric dimers in the absence of flagellin. J. Struct. Biol., 177:402-409.
  24. Liu XQ, and Wang HW*. (2011) Single particle electron microscopy reconstruction of the exosome complex using the random conical tilt method. J. Vis. Exp., doi: 10.3791/2574.
  25. Wu X, Shen QT, Stokes N, Lu C, Zheng Q, Polak L, Wang HW, and Fuchs E*. (2011) Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3β. Cell, 144:341-352.
  26. Wang HW*, Noland C, Siridechadilok B, Taylor DW, Ma E, Felderer K, Doudna JA*, and Nogales E*. (2009) Structural insights into RNA processing by the human RISC-loading complex. Nat. Struct. Mol. Biol., 16:1148-1153.
  27. Wang HW, Long S, Ciferri C, Westermann S, Drubin DG, Barnes G, and Nogales E*. (2008) Architecture and flexibility of the yeast Ndc80 kinetochore complex. J. Mol. Biol., 383:894-903.
  28. Wang HW*, Wang J, Ding F, Callahan K, Bratkowski MA, Bulter JS, Nogales E, and Ke A*. (2007) Architecture of the yeast Rrp44-exosome complex suggests routes of RNA recruitment for 3’-end processing. Proc. Natl. Acad. Sci. USA, 104:16844-16849.
  29. Wang HW, Ramey VH, Westermann S, Leschziner A, Welburn JPI, Nakajima Y, Drubin DG, Barnes G, and Nogales E*. (2007) Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanisms. Nat. Struct. Mol. Biol., 14:721-726.
  30. Westermann W, Wang HW, Avila-Sakar A, Drubin DG, Nogales E, and Barnes G*. (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature, 440:565-569.
  31. Wang HW, and Nogales E*. (2005) The nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly. Nature, 435:911-915.
  32. Mei K, Li Y, Wang S, Shao G, Wang J, Ding Y, Luo G, Yue P, Liu JJ, Wang X, Dong MQ, Wang HW*, Guo W*. (2018) Cryo-EM structure of the exocyst complex. Nat. Struct. Mol. Biol., 25(2):139-146
  33. Fan X, Zhao L, Liu C, Zhang JC, Fan K, Yan X, Peng HL, Lei J*, Wang HW*. (2017) Near-Atomic Resolution Structure Determination in Over-Focus with Volta Phase Plate by Cs-Corrected Cryo-EM. Structure, 25:1623-1630.


Tel: +86-10-62772390 (office)

       +86-10-62798651 (lab)

E-mail: hongweiwang@tsinghua.edu.cn

Lab website: http://cryoem.life.tsinghua.edu.cn/


Tsinghua University,Beijing,China,100084
Tel:+86-10-62788604  Fax:+86-10-62788604  Email:admin-life@biomed.tsinghua.edu.cn